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水平和垂直重力条件下的蛋白质组学分析

Proteomic Analysis of under Conditions of Horizontal and Perpendicular Gravity.

作者信息

Kim Ji-Su, Kwon Young-Sang, Bae Dong-Won, Kwak Youn-Sig, Kwack Yong-Bum

机构信息

Division of Applied Life Science (BK21 Plus) and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Korea.

Environmental Biology & Chemistry Center, Korea Institute of Toxicology (KIT), Jinju 52834, Korea.

出版信息

Mycobiology. 2017 Sep;45(3):226-231. doi: 10.5941/MYCO.2017.45.3.226. Epub 2017 Sep 30.

DOI:10.5941/MYCO.2017.45.3.226
PMID:29138630
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5673521/
Abstract

was employed to investigate the fungal response to gravity. Mycelium growth revealed a consistent growth pattern, irrespective of the direction of gravity (i.e., horizontal vs. perpendicular). However, the fruiting body grew in the direction opposite to that of gravity once the primordia had formed. For the proteomic analysis, only curved-stem samples were used. Fifty-one proteins were identified and classified into 13 groups according to function. The major functional groups were hydrolases and transferases (16%), signal transduction (15%), oxidoreductases and isomerases (11%), carbohydrate metabolism (9%), and transport (5%). To the best of our knowledge, this is the first report on a proteomic approach to evaluate the molecular response of to gravity.

摘要

被用于研究真菌对重力的反应。菌丝体生长呈现出一致的生长模式,与重力方向无关(即水平方向与垂直方向)。然而,一旦原基形成,子实体就会朝着与重力相反的方向生长。对于蛋白质组学分析,仅使用了弯曲茎样本。鉴定出51种蛋白质,并根据功能分为13组。主要功能组为水解酶和转移酶(16%)、信号转导(15%)、氧化还原酶和异构酶(11%)、碳水化合物代谢(9%)以及转运(5%)。据我们所知,这是关于用蛋白质组学方法评估[此处原文缺失相关内容]对重力的分子反应的首次报道。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ba/5673521/421533bb0965/mb-45-226-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ba/5673521/e5ca005fd9bb/mb-45-226-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ba/5673521/5ae92bcd9fb9/mb-45-226-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ba/5673521/421533bb0965/mb-45-226-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ba/5673521/e5ca005fd9bb/mb-45-226-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ba/5673521/5ae92bcd9fb9/mb-45-226-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ba/5673521/421533bb0965/mb-45-226-g003.jpg

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本文引用的文献

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Comparative transcriptomics of the model mushroom Coprinopsis cinerea reveals tissue-specific armories and a conserved circuitry for sexual development.模式蘑菇灰盖鬼伞的比较转录组学揭示了组织特异性防御机制和性发育的保守调控途径。
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Proteomic analysis of Rhizoctonia solani AG-1 sclerotia maturation.根肿菌 AG-1 菌核成熟过程的蛋白质组学分析。
Fungal Biol. 2014 May-Jun;118(5-6):433-43. doi: 10.1016/j.funbio.2014.02.001. Epub 2014 Feb 26.
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重力感知与信号传导。
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Plant Cell. 2002 Jan;14(1):47-56. doi: 10.1105/tpc.010216.
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Life history and developmental processes in the basidiomycete Coprinus cinereus.灰盖鬼伞担子菌的生活史及发育过程
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Genetic evidence that the endodermis is essential for shoot gravitropism in Arabidopsis thaliana.关于拟南芥中内皮层对地上部向重力性至关重要的遗传学证据。
Plant J. 1998 May;14(4):425-30. doi: 10.1046/j.1365-313x.1998.00137.x.
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Compatibility and stability of diploids in Coprinus lagopus.鬼伞属中双倍体的兼容性与稳定性
Genet Res. 1966 Aug;8(1):61-72. doi: 10.1017/s0016672300009915.