Laboratorio Biodiversità dei Funghi, Dipartimento di Biologia Ambientale, Sapienza Università di Roma, 00185, Rome, Italy.
Consiglio per la Ricerca e l'analisi dell'Economia Agraria, Centro di ricerca Agricoltura e Ambiente (CREA-AA), 00184, Rome, Italy.
Appl Microbiol Biotechnol. 2018 Jan;102(2):1019-1033. doi: 10.1007/s00253-017-8614-9. Epub 2017 Nov 14.
The saprotrophic fungus Penicillium griseofulvum was chosen as model organism to study responses to a mixture of hexachlorocyclohexane (HCH) isomers (α-HCH, β-HCH, γ-HCH, δ-HCH) and potentially toxic metals (vanadium, lead) in solid and liquid media. The P. griseofulvum FBL 500 strain was isolated from polluted soil containing high concentrations of HCH isomers and potentially toxic elements (Pb, V). Experiments were performed in order to analyse the tolerance/resistance of this fungus to xenobiotics and to shed further light on fungal potential in inorganic and organic biotransformations. The aim was to examine the ecological and bioremedial potential of this fungus verifying the presence of mechanisms that allow it to transform HCH isomers and metals under different extreme test conditions. To our knowledge, this work is the first to provide evidence on the biotransformation of HCH mixtures, in combination with toxic metals, by a saprotrophic non-white-rot fungus and on the metabolic synergies involved.
选用腐生真菌灰绿青霉作为模式生物，研究其在固体和液体培养基中对六氯环己烷（HCH）异构体（α-HCH、β-HCH、γ-HCH、δ-HCH）和潜在有毒金属（钒、铅）混合物的反应。P. griseofulvum FBL 500 菌株是从含有高浓度 HCH 异构体和潜在有毒元素（Pb、V）的污染土壤中分离出来的。进行这些实验是为了分析该真菌对异生物和无机和有机生物转化中真菌潜在性的耐受性/抗性。目的是检验该真菌的生态和生物修复潜力，验证其在不同极端测试条件下转化 HCH 异构体和金属的机制的存在。据我们所知，这项工作首次提供了关于一种腐生非白腐真菌对 HCH 混合物与有毒金属结合的生物转化的证据，以及所涉及的代谢协同作用。
