Chai Wei, Zhang Wenlong, Wei Zhentong, Xu Ying, Shi Jian, Luo Xiaomei, Zeng Jianying, Cui Manhua, Li Jiannan, Niu Lizhi
Department of Gynecology and Obstetrics, The Second Hospital of Jilin University, 218 Ziqiang Street, Nanguan District, Changchun, Jilin Province, 130041, China.
Department of Gynecology and Obstetrics, The First Hospital of Jilin University, Changchun, 130021, China.
Biomed Microdevices. 2017 Nov 14;19(4):103. doi: 10.1007/s10544-017-0248-2.
The aim of this study was to evaluate the effects of irreversible electroporation (IRE) on the uterine cervix in a rabbit model. IRE ablation was performed in the cervices of 48 New Zealand rabbits, with one ablation lesion in each animal. Gross pathology, transmission electron microscopy, hematoxylin and eosin (H&E), Masson's trichrome (MT) stain, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed at pre-set time points (0 h, 12 h, 1 d, 2 d, 4 d, 7 d, 14 d, and 28 d post-IRE). All the rabbits tolerated the IRE ablation without serious complications. IRE caused complete cell death of the ablated cervix via cell apoptosis. However, fast recovery of the cervix was observed from 7 d post-IRE, with the signs of collagen fibers hyperplasia, the disappearance of the necrotic cells and muscle fibers, and regeneration and extension of the cervical epithelium. At 28 d post-IRE, the ablated cervices recovered to almost normal. Our study suggested that IRE might be an efficient and safe technology to treat cervical tumors, without causing serious cervical damage.
本研究的目的是在兔模型中评估不可逆电穿孔（IRE）对子宫颈的影响。对48只新西兰兔的子宫颈进行IRE消融，每只动物有一个消融灶。在预设时间点（IRE后0小时、12小时、1天、2天、4天、7天、14天和28天）进行大体病理学、透射电子显微镜检查、苏木精-伊红（H&E）染色、Masson三色（MT）染色和末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）分析。所有兔子均耐受IRE消融，无严重并发症。IRE通过细胞凋亡导致消融的子宫颈细胞完全死亡。然而，在IRE后7天观察到子宫颈快速恢复，出现胶原纤维增生、坏死细胞和肌纤维消失以及子宫颈上皮再生和延伸的迹象。在IRE后28天，消融的子宫颈几乎恢复正常。我们的研究表明，IRE可能是一种治疗宫颈肿瘤的有效且安全的技术，不会造成严重的宫颈损伤。
