Komori M, Shimada H, Miura T, Kamataki T
Division of Analytical Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan.
Biochem Pharmacol. 1989 Jan 15;38(2):235-40. doi: 10.1016/0006-2952(89)90032-4.
P-450-male is a male specific form of cytochrome P-450 in rat liver microsomes. Cytochrome P-450 crossreactive with anti-P-450-male antibodies was purified to an electrophoretical homogeneity from liver microsomes of male beagle dogs. The specific content of the purified cytochrome P-450 (P-450-D1) was 16.9 nmol/mg protein. The apparent monomeric molecular weight of P-450-D1 was 48,000, which was smaller than P-450-male (51,000). P-450-D1 showed similarities in spectral properties, N-terminal amino acid sequence, and catalytic activities with some limited exceptions: P-450-D1 did not catalyze 2 alpha-hydroxylation of testosterone and progesterone and catalyzed 21-hydroxylation of progesterone. Based on these results, we propose that P-450-D1 is a form of cytochrome P-450 in the same gene subfamily as P-450-male.
P-450-雄性是大鼠肝微粒体中一种雄性特异性形式的细胞色素P-450。与抗P-450-雄性抗体发生交叉反应的细胞色素P-450从雄性比格犬的肝微粒体中纯化至电泳纯。纯化的细胞色素P-450（P-450-D1）的比含量为16.9 nmol/mg蛋白质。P-450-D1的表观单体分子量为48,000，比P-450-雄性（51,000）小。P-450-D1在光谱特性、N端氨基酸序列和催化活性方面表现出相似性，但有一些有限的例外：P-450-D1不催化睾酮和孕酮的2α-羟基化，却催化孕酮的21-羟基化。基于这些结果，我们提出P-450-D1是与P-450-雄性属于同一基因亚家族的一种细胞色素P-450形式。
