Kunder Christian A, Roncador Giovanna, Advani Ranjana H, Gualco Gabriela, Bacchi Carlos E, Sabile Jean M, Lossos Izidore S, Nie Kexin, Tibshirani Robert John, Green Michael R, Alizadeh Ash A, Natkunam Yasodha
Department of Pathology.
Lymphoma Group, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain.
Am J Clin Pathol. 2017 Nov 20;148(6):465-476. doi: 10.1093/ajcp/aqx099.
KLHL6 is a recently described BTB-Kelch protein with selective expression in lymphoid tissues and is most strongly expressed in germinal center B cells.
Using gene expression profiling as well as immunohistochemistry with an anti-KLHL6 monoclonal antibody, we have characterized the expression of this molecule in normal and neoplastic tissues. Protein expression was evaluated in 1,058 hematopoietic neoplasms.
Consistent with its discovery as a germinal center marker, KLHL6 was positive mainly in B-cell neoplasms of germinal center derivation, including 95% of follicular lymphomas (106/112). B-cell lymphomas of non-germinal center derivation were generally negative (0/33 chronic lymphocytic leukemias/small lymphocytic lymphomas, 3/49 marginal zone lymphomas, and 2/66 mantle cell lymphomas).
In addition to other germinal center markers, including BCL6, CD10, HGAL, and LMO2, KLHL6 immunohistochemistry may prove a useful adjunct in the diagnosis and future classification of B-cell lymphomas.
KLHL6是一种最近被描述的BTB - Kelch蛋白，在淋巴组织中选择性表达，在生发中心B细胞中表达最强。
利用基因表达谱以及抗KLHL6单克隆抗体进行免疫组织化学，我们已对该分子在正常组织和肿瘤组织中的表达进行了特征分析。在1058例造血系统肿瘤中评估了蛋白表达情况。
与其作为生发中心标志物的发现一致，KLHL6主要在生发中心来源的B细胞肿瘤中呈阳性，包括95%的滤泡性淋巴瘤（106/112）。非生发中心来源的B细胞淋巴瘤通常为阴性（0/33例慢性淋巴细胞白血病/小淋巴细胞淋巴瘤、3/49例边缘区淋巴瘤和2/66例套细胞淋巴瘤）。
除了其他生发中心标志物，包括BCL6、CD10、HGAL和LMO2外，KLHL6免疫组织化学可能在B细胞淋巴瘤的诊断及未来分类中证明是一种有用的辅助手段。
