Bennett B A, Clodfelter J, Sundberg D K, Morris M
Department of Physiology and Pharmacology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103.
Am J Hypertens. 1989 Jan;2(1):46-51. doi: 10.1093/ajh/2.1.46.
To investigate the central nervous system (CNS) changes in the spontaneously hypertensive rat (SHR), a tissue culture model was used to examine the content and release (24 hour) of the peptide hormones, vasopressin (VP) and oxytocin (OT), from brain explants. Nuclear regions consisting of the paraventricular (PVN) or supraoptic (SON) nuclei were microdissected from prehypertensive SHR and Wistar-Kyoto (WKY) rats. Media levels of VP and OT were measured at 1, 3, 4, and 7 days of culture. After three days of culture, the PVN explants from SHR secreted significantly less VP and OT (both reduced 80%) than did those from WKY. Release of both VP and OT in the SON explants was significantly lower (approximately 50% lower) in the SHR only at seven days of culture. Additionally, tissue content of the peptides was measured after 0, 1, 4, and 7 days of culture. Tissue content of VP and OT was decreased (40% or more) in the SHR in both nuclear regions after four and seven days of culture. In addition, nicotine was found to stimulate the release of VP from SON, but not PVN, cultures in both SHR and WKY explants. Immunohistochemical data showed that there was not a preferential loss of VP or OT neurons in explants from the SHR. Therefore, this in vitro model would indicate that there is a difference in the ability of cultured explants of PVN and SON from SHR and WKY (four-week-old) to synthesize and/or release the peptide hormones VP and OT.
为了研究自发性高血压大鼠（SHR）中枢神经系统（CNS）的变化，采用组织培养模型检测脑外植体中肽类激素血管加压素（VP）和催产素（OT）的含量及释放情况（24小时）。从高血压前期的SHR和Wistar-Kyoto（WKY）大鼠中显微解剖出包含室旁核（PVN）或视上核（SON）的核区域。在培养的第1、3、4和7天测量培养基中VP和OT的水平。培养三天后，SHR的PVN外植体分泌的VP和OT明显少于WKY的PVN外植体（两者均减少80%）。仅在培养七天时，SHR的SON外植体中VP和OT的释放量显著降低（约低50%）。此外，在培养0、1、4和7天后测量肽类的组织含量。培养四天和七天后，SHR两个核区域中VP和OT的组织含量均降低（40%或更多）。此外，发现尼古丁可刺激SHR和WKY外植体中SON培养物释放VP，但不能刺激PVN培养物释放VP。免疫组织化学数据显示，SHR外植体中VP或OT神经元没有优先丢失。因此，该体外模型表明，SHR和WKY（四周龄）的PVN和SON培养外植体在合成和/或释放肽类激素VP和OT的能力上存在差异。
