Hutchinson F J, Francis S E, Lyle I G, Jones M N
Department of Biochemistry & Molecular Biology, University of Manchester, U.K.
Biochim Biophys Acta. 1989 Jan 16;978(1):17-24. doi: 10.1016/0005-2736(89)90492-6.
The problem of characterising liposomes with covalently attached proteins has been analysed theoretically in terms of a normal weight distribution of liposome diameters. The polydispersity of protein conjugation is considered in terms of the width (standard deviation) of the liposome size distribution. It is shown that the weight-average number of proteins per liposome is a convenient parameter to use to define the protein content of proteoliposomes. Two types of proteoliposome have been prepared (small unilamellar vesicles and reverse phase evaporation vesicles) in which wheat germ agglutinin is covalently coupled to the liposomal surface. The liposomes cover a range of weight average diameter from 65 to 240 nm and of polydispersity (weight to number average diameter (dw/dn) from 2.6 to 11.4. The liposomes have been characterised by chemical analysis and photon correlation spectroscopy and the results are discussed in terms of the theoretical consequences of an equivalent normal weight distribution of diameters.
已根据脂质体直径的正态重量分布从理论上分析了表征共价连接蛋白质的脂质体的问题。蛋白质缀合的多分散性是根据脂质体大小分布的宽度（标准差）来考虑的。结果表明，每个脂质体的重均蛋白质数量是用于定义蛋白脂质体蛋白质含量的一个方便参数。已制备了两种类型的蛋白脂质体（小单层囊泡和反相蒸发囊泡），其中麦胚凝集素共价偶联到脂质体表面。这些脂质体的重均直径范围为65至240 nm，多分散性（重量平均直径与数量平均直径之比(dw/dn)）为2.6至11.4。通过化学分析和光子相关光谱对脂质体进行了表征，并根据直径等效正态重量分布的理论结果对结果进行了讨论。
