Rosenstein J M, Krum J M, Trapp B D
Department of Anatomy, George Washington University Medical Center, Washington, DC 20037.
Brain Res. 1989 Jan 2;476(1):110-9. doi: 10.1016/0006-8993(89)91542-4.
Autonomic (superior cervical) ganglia were grafted either into the IV ventricle where minimal trauma occurred or directly into the cerebral cortex which was necessarily traumatic. Previous studies have shown that host astroglia may migrate into autonomic tissue grafts. The purpose of the present study was to compare and contrast the astroglial response in allo- and autografts. By monitoring the host response in the two model sites using glial fibrillary acidic protein (GFAP) immunostaining in 1 micron plastic sections we sought to determine the role of injury stimulus in astroglial migration. In addition, these models could be used to investigate any potential differences in glial reactivity produced by allo- or autograft antigenic stimulation. In both ventricular and parenchymal locations, astroglia migrated progressively into allografts. Migration, which could have taken place along anastomotic vascular connections, began after one week and was continual, eventually replacing graft neural tissue. Astrocytic processes appeared enlarged and highly immunoreactive only as they entered the allografts or were in close association with the choroid plexus; adjacent host astrocytes were unaltered. Glial migration was greatly reduced in ventricular autografts but in the parenchymal site was nearly comparable to that of allografts. It was suggested that certain immunological factors may be involved in glial reactivity or migration considering the observed differences in the non-traumatic model whereas tissue damage stimulus played a major role in migration in both allo- and autografts. In no instances were typical astrocytic end-feet found on the autonomic graft vessels. The host astrocytic response to grafted autonomic tissue occurred significantly later (5-7 days) than the host endothelial response. This observation indicates that the graft vessels were original, intrinsic ones and the astrocytic invasion played no role in influencing endothelium with regards to brain-barrier properties.
