Yamazaki Kohei, Kashimoto Takashige, Hashimoto Yuki, Kado Takehiro, Ueno Shunji
Laboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, Higashi 23-35-1, Towada, Aomori 034-8628, Japan.
J Vet Med Sci. 2018 Jan 1;80(1):55-58. doi: 10.1292/jvms.17-0395. Epub 2017 Nov 15.
Vibrio vulnificus is known as an opportunistic bacterial pathogen that causes primary septicemia and wound infection in humans. Recently, the incidence of wound infection by V. vulnificus is increasing in warm countries. In this study, we examined a vaccine antigen against V. vulnificus in mice. FlaB, a component protein of the V. vulnificus flagellum, was expressed as a recombinant protein, named rFlaB. After immunization of mice with rFlaB, the mice were challenged by subcutaneous inoculation with V. vulnificus. Bacterial burdens in muscular tissue at the infection site in rFlaB-immunized mice were significantly decreased compared with those of control mice. We found that rFlaB immunization can partially suppress proliferation of V. vulnificus at the local infection site.
创伤弧菌是一种机会性细菌病原体，可导致人类原发性败血症和伤口感染。最近，在温暖的国家，创伤弧菌引起的伤口感染发病率正在上升。在本研究中，我们在小鼠中检测了一种针对创伤弧菌的疫苗抗原。创伤弧菌鞭毛的组成蛋白FlaB被表达为重组蛋白，命名为rFlaB。用rFlaB免疫小鼠后，通过皮下接种创伤弧菌对小鼠进行攻击。与对照小鼠相比，rFlaB免疫小鼠感染部位肌肉组织中的细菌载量显著降低。我们发现rFlaB免疫可以部分抑制创伤弧菌在局部感染部位的增殖。
