染色体片段化：一种用于筛选高匙羹藤酸产量的匙羹藤（R. Br.）种质的可能标记

Chromosomal Fragmentation: A Possible Marker for the Selection of High Gymnemic Acid Yielding Accessions of R. Br.

作者信息

Verma Ashutosh Kumar, Dhawan Sunita Singh

机构信息

Central Botanical Laboratory, Botanical Survey of India, AJC Bose Indian Botanic Garden, Howrah, West Bengal, India.

Plant Biotechnology Division, CSIR - Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, India.

出版信息

Pharmacogn Mag. 2017 Oct;13(Suppl 3):S481-S483. doi: 10.4103/pm.pm_420_16. Epub 2017 Oct 11.

DOI:10.4103/pm.pm_420_16

PMID:29142402

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5669085/

Abstract

BACKGROUND

R. Br. a member of family Asclepiadaceae as mentioned in Indian Pharmacopoeia popular among the researchers because of stimulatory effect of its phytoconstituent on pancreatic cells and potential to treat Type I and II type of diabetes.

OBJECTIVES

Development of cost-effective marker system for the selection of high gymnemic acid yielding accessions of .

MATERIALS AND METHODS

Presoaked seeds of treated with different dilutions of gymnemagenin and 10% leaf extract of twenty different accessions of . Root tips of germinated seeds were fixed, and chromosomal studies were made by root tip bioassay method.

RESULTS

Exposure of seeds to treatment solutions promotes various types of chromosomal anomalies in root meristem, and surprisingly, direct correlation between the percentage of chromosomal fragmentation and the percentage of gymnemic acid shared by treatment solution were observed.

CONCLUSION

Later finding may be explored for the development of a novel methodology or marker system for the selection of high active principle yielding accessions of .

SUMMARY

An experiment was carried out using root tip bioassay method for the study of effect of different dilutions of standard gymnemic acid and 10% leaf extract of twenty different accessions of on root tip meristem of . Various types of chromosomal anomalies were observed. Of which, percentage of chromosomal fragmentation was showed a direct (∞) relationship with the percentage of gymnemic acid shared by treatment solution. This interesting result after more and more exploration and revalidation could be utilized for the development of a novel methodology for the selection of high active principle yielding accessions of . MI: Mitotic index; CP: Condensed prophase; CM: Clumped metaphase; MC: Metaphase cleft; FR: Fragmentation; AP: Anaphase with persistent nucleolous; LA: Laggard, BR: Bridge; BI: Bi-nucleated cell; DA: Disturbed anaphasic polarity.

摘要

背景

萝藦科的匙羹藤如《印度药典》所述，因其植物成分对胰腺细胞的刺激作用以及治疗I型和II型糖尿病的潜力而受到研究人员的广泛关注。

目的

开发一种经济高效的标记系统，用于筛选匙羹藤中高匙羹藤酸产量的种质。

材料与方法

用不同稀释度的匙羹藤皂苷元处理匙羹藤预先浸泡的种子，并使用20种不同匙羹藤种质的10%叶提取物。将发芽种子的根尖固定，采用根尖生物测定法进行染色体研究。

结果

种子暴露于处理溶液会促进根分生组织中出现各种类型的染色体异常，令人惊讶的是，观察到染色体断裂百分比与处理溶液中匙羹藤酸百分比之间存在直接相关性。

结论

后续研究可探索利用这一发现开发一种新方法或标记系统，用于筛选匙羹藤中高活性成分产量的种质。

总结

采用根尖生物测定法进行实验，研究不同稀释度的标准匙羹藤酸和20种不同匙羹藤种质的10%叶提取物对匙羹藤根尖分生组织的影响。观察到各种类型的染色体异常。其中，染色体断裂百分比与处理溶液中匙羹藤酸百分比呈直接（∞）关系。经过越来越多的探索和重新验证，这一有趣的结果可用于开发一种新方法，以筛选匙羹藤中高活性成分产量的种质。MI：有丝分裂指数；CP：凝缩前期；CM：聚团中期；MC：中期裂隙；FR：断裂；AP：带有持续核仁的后期；LA：落后染色体；BR：桥；BI：双核细胞；DA：后期极性紊乱。