Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University , Suzhou, Jiangsu 215007, P. R. China.
Yancheng City No. 1 People's Hospital, the Fourth Affiliated Hospital of Nantong University , Yancheng, Jiangsu 224001, P. R. China.
ACS Appl Mater Interfaces. 2017 Nov 29;9(47):41168-41180. doi: 10.1021/acsami.7b13167. Epub 2017 Nov 16.
Periosteum plays the pivotal role in neomineralization, vascularization and protection during bone tissue regeneration. However, many artificial periosteum focused only on protection and lacked of the osteogenesis and angiogenesis functional capacity. In this study, we developed a novelty inorganic strengthened gelatin hydrogel membrane via inorganic and organic co-cross-linked double network as artificial periosteum for enhancing the durable angiogenesis and osteogenesis in bone reconstruction. Mesoporous bioactive glass nanoparticles (MBGNs) chemically modified with photo-cross-linkable gelatin derivative (GelMA) were further incorporated into GelMA to fabricate an organic/inorganic co-cross-linked hydrogel membrane (GelMA-G-MBGNs). The GelMA-G-MBGNs hydrogel membrane displayed better mechanical property, durable degradation time, pH stable, biomineralization and long-term ion release. In vitro study demonstrated that, when compared with GelMA or GelMA/MBGNs, the GelMA-G-MBGN membrane significantly promoted osteogenic differentiation while maintaining stable local pH, which is conducive to cell adhesion and proliferation. Finally, the GelMA-G-MBGN membrane shows a superior artificial periosteum with superior capacity in angiogenesis and osteogenesis for accelerating new and mature lamellar bone formation in rat calvarial critical size defect. This co-cross-linked hydrogel membrane implied a promising strategy for the development of advanced periosteum biomaterials with excellent handle and bone repairing properties.
骨膜在组织再生过程中的新矿化、血管生成和保护中起着关键作用。然而，许多人工骨膜仅注重保护，缺乏成骨和血管生成的功能能力。在这项研究中，我们通过无机和有机共交联双网络开发了一种新型无机强化明胶水凝胶膜作为人工骨膜，以增强骨重建中的持久血管生成和成骨作用。进一步将经化学修饰的具有光交联明胶衍生物（GelMA）的介孔生物活性玻璃纳米颗粒（MBGNs）掺入 GelMA 中，制备有机/无机共交联水凝胶膜（GelMA-G-MBGNs）。GelMA-G-MBGNs 水凝胶膜具有更好的机械性能、持久的降解时间、稳定的 pH 值、生物矿化和长期离子释放。体外研究表明，与 GelMA 或 GelMA/MBGNs 相比，GelMA-G-MBGN 膜显著促进成骨分化，同时保持稳定的局部 pH 值，有利于细胞黏附和增殖。最后，GelMA-G-MBGN 膜表现出优异的人工骨膜性能，具有出色的血管生成和成骨能力，可加速大鼠颅骨临界尺寸缺损中新的和成熟的板层骨形成。这种共交联水凝胶膜为开发具有良好操作性和骨修复性能的先进骨膜生物材料提供了一种有前景的策略。
