Department of Applied Chemistry, SV National Institute of Technology (SVNIT), Surat 395007, India.
Dipartimento di Scienze Chimiche e Geologiche, Università di Cagliari, 09042 Monserrato, Italy.
Biosens Bioelectron. 2018 Apr 15;102:196-203. doi: 10.1016/j.bios.2017.11.031. Epub 2017 Nov 9.
The present work reports the interaction of various vitamin B cofactors with the red emitting glutathione stabilized copper nanoclusters (GSH-CuNCs). Addition of pyridoxamine (PM) resulted a new turn-on band at 410nm due to the possible adsorption over the surface of GSH-CuNCs. The nano-assembly PM-GSH-CuNCs was applied for the selective detection of nitro-aromatic compounds. Upon addition of picric acid (PA), the fluorescence of PM-GSH-CuNCs was selectively quenched at 410nm and ~ 625nm among the other tested nitro-aromatic compounds. With a linearity range from 9.9μM to 43μM, the concentration of PA can be detected down to 2.74μM. The high selectivity exhibited by the nano-assembly allows to detect PA in real samples like tap water, river water and matchstick. Advantageously, the nano-assembly PM-GSH-CuNCs was chemically adsorbed over the cellulosic strips and applied for the naked-eye detection of PA down to 1μM.
本工作研究了各种维生素 B 辅助因子与红色发射谷胱甘肽稳定的铜纳米团簇（GSH-CuNCs）的相互作用。由于 PM 可能吸附在 GSH-CuNCs 的表面上，所以加入吡哆醛（PM）后在 410nm 处出现新的开启带。纳米组装 PM-GSH-CuNCs 被用于选择性检测硝基芳香族化合物。在加入苦味酸（PA）后，PM-GSH-CuNCs 的荧光在 410nm 和~625nm 处被其他测试的硝基芳香族化合物选择性猝灭。该纳米组装物的线性范围从 9.9μM 到 43μM，可以检测到低至 2.74μM 的 PA 浓度。纳米组装物表现出的高选择性允许在实际样品（如自来水、河水和火柴棒）中检测 PA。有利的是，纳米组装 PM-GSH-CuNCs 被化学吸附在纤维素条上，并用于检测低至 1μM 的 PA 的肉眼检测。
