Ahnert-Hilger G, Weller U, Dauzenroth M E, Habermann E, Gratzl M
Abteilung Anatomie und Zellbiologie, Universität Ulm, FRG.
FEBS Lett. 1989 Jan 2;242(2):245-8. doi: 10.1016/0014-5793(89)80478-8.
The intracellular action on exocytosis of various forms of tetanus toxin was studied using adrenal medullary chromaffin cells, the membrane barrier of which has been removed by permeabilization with streptolysin O. Such cells still release catecholamines on stimulation with calcium. The two-chain form of tetanus toxin (67 nmol/l) strongly inhibited exocytosis, but only if dithiothreitol was present as a reducing agent. Purified light chain completely prevented [3H]noradrenaline release with a half-maximal effect at about 5 nmol/l. Heavy chain (up to 11 nmol/l) and unprocessed single-chain toxin (up to 133 nmol/l) were without effect. It is concluded that the original single-chain form of tetanus toxin has to be processed by proteolysis and reduction to yield a light chain which inhibits transmitter release.
利用肾上腺髓质嗜铬细胞研究了各种形式破伤风毒素对胞吐作用的细胞内效应，该细胞的膜屏障已通过用链球菌溶血素O透化处理而去除。这类细胞在受到钙刺激时仍会释放儿茶酚胺。破伤风毒素的双链形式（67 nmol/l）强烈抑制胞吐作用，但前提是存在二硫苏糖醇作为还原剂。纯化的轻链完全阻止了[3H]去甲肾上腺素的释放，在约5 nmol/l时达到半数最大效应。重链（高达11 nmol/l）和未加工的单链毒素（高达133 nmol/l）则没有作用。得出的结论是，破伤风毒素的原始单链形式必须经过蛋白水解和还原处理才能产生抑制递质释放的轻链。
