Kasten F H, Pineda L F, Schneider P E, Rawls H R, Foster T A
Department of Anatomy, Louisiana State University School of Dentistry, New Orleans 70119.
In Vitro Cell Dev Biol. 1989 Jan;25(1):57-62. doi: 10.1007/BF02624411.
Cell culture is a valuable method of evaluating the biocompatibility of new dental materials. The purpose of this study was to compare the in vitro biocompatibility of an experimental fluoride composite resin with fluoride and non-fluoride-releasing materials currently available. The dental materials tested were: MQ Silicate (silicate cement), KETAC-CEM and FUJI (type II glass ionomer cements), VISIO DISPERS (a light-cured, nonfluoridated, microfilled composite resin), and FR-17 (an experimental fluoride-releasing composite resin). The Smulow-Glickman (S-G) human gingival epithelial cell line, which exhibits semidifferentiated characteristics, was used in the study as a test system. Biocompatibility was quantified by counting the viable cells per unit area remaining after 24 and 48 h at two radial distances from cured specimens immersed in the cell culture medium. The test materials were observed to be most toxic to cells nearest the materials. A Time-Distance Cytotoxicity Index (TDCI) was calculated to relate the percentage of dead cells to viable cells at each diffusion distance for each exposure time compared to a nontoxic control. The relative toxicity ranking of the materials tested based on the TDCI was VISIO DISPERS (91%), FUJI (82%), FR-17 (30%), MQ Silicate (23%), and KETAC-CEM (10%), which exhibited the least toxicity. The cytotoxicity of the experimental resin FR-17 was within the range of cytotoxicity of currently accepted restorative materials.
细胞培养是评估新型牙科材料生物相容性的一种重要方法。本研究的目的是比较一种实验性含氟复合树脂与目前市售的含氟和不含氟材料的体外生物相容性。所测试的牙科材料有：MQ硅酸盐（硅酸盐水泥）、KETAC - CEM和FUJI（II型玻璃离子水门汀）、VISIO DISPERS（一种光固化、不含氟的微填料复合树脂）以及FR - 17（一种实验性含氟复合树脂）。本研究采用表现出半分化特征的Smulow - Glickman（S - G）人牙龈上皮细胞系作为测试系统。通过对浸入细胞培养基中的固化标本在两个径向距离处24小时和48小时后每单位面积剩余的活细胞进行计数来量化生物相容性。观察到测试材料对距离材料最近的细胞毒性最大。计算了一个时间 - 距离细胞毒性指数（TDCI），以将每个暴露时间在每个扩散距离处的死细胞百分比与活细胞百分比与无毒对照进行比较。根据TDCI对测试材料的相对毒性排名为：VISIO DISPERS（91%）、FUJI（82%）、FR - 17（30%）、MQ硅酸盐（23%）和KETAC - CEM（10%），后者毒性最小。实验树脂FR - 17的细胞毒性在目前公认的修复材料细胞毒性范围内。
