Jacobs N J, Borotz S E, Guerinot M L
Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03756.
J Bacteriol. 1989 Jan;171(1):573-6. doi: 10.1128/jb.171.1.573-576.1989.
Extracts of the crude bacteroid fraction of symbiotically grown Bradyrhizobium japonicum were much more active in oxidizing protoporphyrinogen to protoporphyrin than were extracts of cells grown under free-living conditions, especially when assayed in atmospheres containing only traces of oxygen. This correlates with the higher heme content of the microaerophilic nodules. Furthermore, the high level of oxidative activity in the crude bacteroid fraction was associated with an uncharacterized membrane fraction, probably of plant origin, that was separable from the bacteroids by Percoll gradient centrifugation.
共生生长的日本慢生根瘤菌的粗类菌体部分提取物在将原卟啉原氧化为原卟啉方面比在自由生活条件下生长的细胞提取物更具活性,尤其是在仅含有微量氧气的气氛中进行测定时。这与微需氧性根瘤中较高的血红素含量相关。此外,粗类菌体部分中的高氧化活性与一种未鉴定的膜部分有关,该膜部分可能源自植物,可通过Percoll梯度离心与类菌体分离。