Delfosse C, Lafont-Lecuelle C, Barthélémy H, Chabaud C, Teruel E, Bienboire-Frosini C, Pageat P
Research Institute in Semiochemistry and Applied Ethology (IRSEA) Route du Chêne, Quartier Salignan, Apt, France.
Research Institute in Semiochemistry and Applied Ethology - Aquaculture Research Centre (IRSEA-ARC), Vikebukt, Norway.
J Fish Dis. 2018 Jun;41(6):969-972. doi: 10.1111/jfd.12747. Epub 2017 Nov 17.
The screening of putative semiochemicals involved in the interaction between Lepeophtheirus salmonis copepodid and the Atlantic salmon could be used to develop methods to prevent infestation. A bioassay was designed to evaluate the attractiveness of Atlantic salmon for L. salmonis copepodids by counting the number of hooked parasites. A salmon was bathed in a tank with 60 copepodids during 45 min. The salmon was humanely killed and its body was scrubbed above a plastic bag. The content of the bag was filtered to count the number of hooked parasites. This procedure was tested at different water temperatures and for different body mass. The temperature significantly influenced the number of hooked copepodids (p < .05). Smolts presented significantly more hooked copepodids at a higher water temperature (24.6 at 13.8°C) compared to smolts at a lower temperature (18.6 at 6.1°C; p = .011). No correlation was found between the body mass and the number of hooked parasites (p = .65). This bioassay was used to successfully measure the attractiveness of Atlantic salmon for L. salmonis copepodids, making it an interesting tool to screen putative semiochemicals designed to prevent the infestation.
筛选参与鲑鳟鱼虱桡足幼体与大西洋鲑鱼相互作用的假定信息素，可用于开发预防感染的方法。设计了一种生物测定法，通过计算附着寄生虫的数量来评估大西洋鲑鱼对鲑鳟鱼虱桡足幼体的吸引力。将一条鲑鱼置于装有60只桡足幼体的水箱中45分钟。对鲑鱼实施安乐死后，在塑料袋上方擦拭其身体。过滤袋中的内容物以计算附着寄生虫的数量。该程序在不同水温及不同体重条件下进行了测试。水温对附着桡足幼体的数量有显著影响（p < 0.05）。与低温（6.1°C时为18.6只）的鲑鱼幼鱼相比，高温（13.8°C时为24.6只）的鲑鱼幼鱼附着的桡足幼体显著更多（p = 0.011）。未发现体重与附着寄生虫数量之间存在相关性（p = 0.65）。该生物测定法成功用于测量大西洋鲑鱼对鲑鳟鱼虱桡足幼体的吸引力，使其成为筛选用于预防感染的假定信息素的有趣工具。
