利用酵母功能筛选系统从旱生盐生植物白滨藜中探索耐盐相关基因。

Exploration for the Salinity Tolerance-Related Genes from Xero-Halophyte Atriplex canescens Exploiting Yeast Functional Screening System.

机构信息

College of Plant Sciences, Jilin University, Changchun 130062, China.

College of Resource and Environment, Jilin Agricultural University, Changchun 130062, China.

出版信息

Int J Mol Sci. 2017 Nov 17;18(11):2444. doi: 10.3390/ijms18112444.

Plant productivity is limited by salinity stress, both in natural and agricultural systems. Identification of salt stress-related genes from halophyte can provide insights into mechanisms of salt stress tolerance in plants. is a xero-halophyte that exhibits optimum growth in the presence of 400 mM NaCl. A cDNA library derived from highly salt-treated plants was constructed based on a yeast expression system. A total of 53 transgenic yeast clones expressing enhanced salt tolerance were selected from 10⁵ transformants. Their plasmids were sequenced and the gene characteristics were annotated using a BLASTX search. Retransformation of yeast cells with the selected plasmids conferred salt tolerance to the resulting transformants. The expression patterns of 28 of these stress-related genes were further investigated in leaves by quantitative reverse transcription-PCR. In this study, we provided a rapid and robust assay system for large-scale screening of genes for varied abiotic stress tolerance with high efficiency in .

植物的生产力受到盐胁迫的限制，无论是在自然系统还是农业系统中都是如此。从盐生植物中鉴定与盐胁迫相关的基因，可以深入了解植物耐盐胁迫的机制。是一种旱生盐生植物，在 400mM NaCl 存在的情况下表现出最佳的生长。根据酵母表达系统，从经过高度盐处理的植物中构建了 cDNA 文库。从 10⁵个转化体中选择了总共 53 个表达增强耐盐性的转基因酵母克隆。对它们的质粒进行测序，并使用 BLASTX 搜索对基因特征进行注释。用所选质粒对酵母细胞进行再转化，赋予了转化体耐盐性。通过定量逆转录-PCR 进一步研究了这些与胁迫相关的 28 个基因在叶片中的表达模式。在这项研究中，我们提供了一个快速而稳健的检测系统，用于在中高效地大规模筛选各种非生物胁迫耐受性基因。