Farahani Parisa, Halabian Raheleh, Vahedi Ensieh, Salimian Jafar
Biology Department, East Tehran Branch, Islamic Azad University, Tehran, Iran.
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2017 Oct;16(5):396-403.
The long lasting inflammation and immune dysregulation is one of the main mechanisms involved in lung complication of veterans exposed to sulfur mustard (SM) gas. Th17/Treg cells have an important role in immunopathogenesis of chronic obstructive pulmonary disease (COPD) and mustard lung disease. In this study, expression of cytokines genes levels related to Th17/Treg cells was determined in peripheral blood mononuclear (PBMC) of mustard lung patients and was compared with COPD patients and healthy controls (HC). Real time-polymerase chain reaction was used to assay genes expression levels of Th17 related cytokines (IL-17, IL-6 and TGF-β) and Treg related cytokines (IL-10, TGF-β). IL-17 gene expression level considerably was higher in SM patients (9.98±0.65, p<0.001), and COPD (4.75±0.71, p<0.001), compare to HC group. Also, gene expression level of IL-6 in the SM group (3.31±0.93, p<0.001) and COPD group (2.93±0.21, p<0.001) were significantly higher than the HC group. The IL-10 gene expression level showed a high increase in SM patients (4.12±0.91, p<0.01), and COPD (2.1±0.45, p<0.01). Finally, the TGF-β gene expression level was increased in SM patients (4.91±0.69, p<0.001) as well as in COPD group (5.41±0.78, p<0.001). In SM patients, IL-17 (R=-0.721, p<0.05), IL-6 (R=-0.621, p<0.05) and TGF-β (R=-0.658, p<0.05) had significant negative association with FEV1 (%). Inversely, Il-10 showed positive correlation (R=0.673) with FEV1 (%). Th17/Treg cells related cytokines genes were highly expressed and imbalanced in peripheral blood mononuclear cells of SM and COPD patients which correlated with pulmonary dysfunction.
长期的炎症和免疫失调是接触芥子气（SM）的退伍军人肺部并发症的主要机制之一。Th17/Treg细胞在慢性阻塞性肺疾病（COPD）和芥子气肺病的免疫发病机制中起重要作用。在本研究中，测定了芥子气肺病患者外周血单个核细胞（PBMC）中与Th17/Treg细胞相关的细胞因子基因水平，并与COPD患者和健康对照（HC）进行比较。采用实时聚合酶链反应检测Th17相关细胞因子（IL-17、IL-6和TGF-β）和Treg相关细胞因子（IL-10、TGF-β）的基因表达水平。与HC组相比，SM患者（9.98±0.65，p<0.001）和COPD患者（4.75±0.71，p<0.001）的IL-17基因表达水平显著更高。此外，SM组（3.31±0.93，p<0.001）和COPD组（2.93±0.21，p<0.001）的IL-6基因表达水平显著高于HC组。IL-10基因表达水平在SM患者（4.12±0.91，p<0.01）和COPD患者（2.1±0.45，p<0.01）中显著升高。最后，TGF-β基因表达水平在SM患者（4.91±0.69，p<0.001）和COPD组（5.41±0.78，p<0.001）中均升高。在SM患者中，IL-17（R=-0.721，p<0.05）、IL-6（R=-0.621，p<0.05）和TGF-β（R=-0.658，p<0.05）与第一秒用力呼气容积（FEV1）（%）呈显著负相关。相反，IL-10与FEV1呈正相关（R=0.673）。Th17/Treg细胞相关细胞因子基因在SM和COPD患者外周血单个核细胞中高表达且失衡，这与肺功能障碍相关。
