Kim Do Hyun, Kim Si Won, Kim Soo Whan, Kang Jun-Myung
Department of Otolaryngology-Head and Neck Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Department of Otolaryngology-Head and Neck Surgery, Bucheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Iran J Allergy Asthma Immunol. 2017 Oct;16(5):404-417.
The purpose of this study was to investigate the effects of interleukin-37 (IL-37) on a Dermatophagoides farinae (Der f)-induced murine model of allergic rhinitis (AR). BALB/c mice, except the control groups, were sensitized intraperitoneally and challenged intranasally with Der f (Der f group). The IL-37 and IL-37+anti-CD25 groups were administered IL-37 intranasally. The IL-37+anti-CD25 groups were administered anti-CD25 monoclonal antibody intraperitoneally before challenge. Allergic symptoms and the average eosinophil number were counted. The levels of cytokines and transcription factors in the nasal mucosa were measured by Real-Time polymerase chain reaction (PCR) and western blotting. The levels of Der f-specific immunoglobulin E (IgE) were measured. The CD4+CD25+Foxp3+T cells among splenic mononuclear cells were analyzed by flow cytometry. The allergic symptom scores and Der f-specific IgE levels were lower in the IL-37 group compared to the Der f group. Additionally, the levels of the transcription factor GATA-3 and ROR-γt and those of the cytokines IL-4, IL-5, IL-13, and IL-17, representing both T helper (Th)2 and Th17 responses, were lower in the IL-37 group in comparison with the Der f group. However, the Th1 responsewas not suppressed after administration of IL-37. IL-37 increased the IL-10 level; however, Real-Time PCR, western blotting, and flow cytometry results showed the limited action of IL-37 on CD4+CD25+Foxp3+T cells. This study demonstrates that intranasal IL-37 can suppress Th2 and Th17 responses in an AR murine model. Furthermore, these data suggest that IL-10 is increased, but CD4+CD25+Foxp3+T cells are not correlated with the IL-37-induced mechanism.
本研究旨在探讨白细胞介素 -37（IL-37）对粉尘螨（Der f）诱导的小鼠变应性鼻炎（AR）模型的影响。除对照组外，BALB/c小鼠经腹腔致敏，并用Der f进行鼻内激发（Der f组）。IL-37组和IL-37 +抗CD25组经鼻给予IL-37。IL-37 +抗CD25组在激发前经腹腔给予抗CD25单克隆抗体。计数变应性症状和平均嗜酸性粒细胞数。通过实时聚合酶链反应（PCR）和蛋白质印迹法检测鼻黏膜中细胞因子和转录因子的水平。检测Der f特异性免疫球蛋白E（IgE）的水平。通过流式细胞术分析脾单个核细胞中的CD4 + CD25 + Foxp3 + T细胞。与Der f组相比，IL-37组的变应性症状评分和Der f特异性IgE水平较低。此外，与Der f组相比，IL-37组中代表辅助性T（Th）2和Th17反应的转录因子GATA-3和ROR-γt以及细胞因子IL-4、IL-5、IL-13和IL-17的水平较低。然而，给予IL-37后Th1反应未受到抑制。IL-37增加了IL-10水平；然而，实时PCR、蛋白质印迹法和流式细胞术结果显示IL-37对CD4 + CD25 + Foxp3 + T细胞的作用有限。本研究表明，鼻内给予IL-37可抑制AR小鼠模型中的Th2和Th17反应。此外，这些数据表明IL-10增加，但CD4 + CD25 + Foxp3 + T细胞与IL-37诱导的机制无关。
