表面筛分配位 IMAC 材料用于纯化 His 标签蛋白。

Surface sieving coordinated IMAC material for purification of His-tagged proteins.

机构信息

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

出版信息

Anal Chim Acta. 2018 Jan 2;997:9-15. doi: 10.1016/j.aca.2017.10.021. Epub 2017 Oct 27.

DOI:10.1016/j.aca.2017.10.021

PMID:29149997

Abstract

Tailor-made materials for the purification of proteins with His-tag was designed through synergizing the selectivity of surface sieving and metal ion affinity. By excluding impurity proteins out of the surface polymer network, such materials could purify His-tagged proteins from the crude cell lysis with purity up to 90%, improved by 14% compared to that obtained by the commercial metal chelating affinity materials. This study might promote the His-tagged protein purification to a new level.

摘要

通过协同利用表面筛滤的选择性和金属离子亲和力，设计了用于纯化带有 His 标签的蛋白质的定制材料。通过将杂质蛋白排除在表面聚合物网络之外，此类材料可从粗细胞裂解物中纯化 His 标记的蛋白质，纯度高达 90%，比商业金属螯合亲和材料提高了 14%。本研究可能将 His 标记的蛋白质纯化推向一个新的水平。