Chen Jiewei, Yang Pengfei, Xiao Yongbo, Zhang Yijun, Liu Jun, Xie Dan, Cai Muyan, Zhang Xinke
Department of pathology, Sun Yat-Sen University Cancer Center, 510060 Guangzhou, China.
State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine.
J Cancer. 2017 Oct 23;8(18):3897-3902. doi: 10.7150/jca.20324. eCollection 2017.
The aim of this study is to investigate the differential expression of α-sma-positive fibroblasts (CAFs) in nasopharyngeal carcinomas (NPCs), nasopharyngitis, metastatic tissues of NPCs and its prognostic value in NPCs. The expression of α-sma-labeled CAFs in 85 NPCs, 32 nasopharyngitis and 12 metastatic tissues of NPCs was detected by immunohistochemical method. The relationship between CAFs and clinicopathological parameters of NPCs was analyzed. The high density of CAFs in the NPCs, nasopharyngitis and metastatic tissues of NPCs group were 41.2% (35/85), 6.2% (2/32) and 83.3% (10/12), and a significant difference was showed among these three groups (<0.05). Chi-square test showed that there was no significant correlation between the density of CAFs and gender, age, N stage, treatment >0.05), but closely correlated with T stage and relapse (<0.05). Kaplan-Meier survival analysis showed that the mean overall survival of high-density and low-density CAFs was 86.8 months and 127.0 months, respectively. Correspondingly, the 5-year survival rates were 57.1% (20/35) and 90.0% (45/50), and there were inversely statistical differences between two groups (<0.05). Cox multivariate analysis showed that the density of CAFs could be used as an independent prognostic factor for the survival of NPC patients (<0.05). The density of CAFs could be closely related to the metastasis of NPCs, and also is an efficient prediction factor of poor survival in patients with NPCs.
本研究旨在探讨α -平滑肌肌动蛋白阳性成纤维细胞（CAFs）在鼻咽癌（NPC）、鼻咽炎、NPC转移组织中的差异表达及其在NPC中的预后价值。采用免疫组化方法检测85例NPC、32例鼻咽炎及12例NPC转移组织中α -平滑肌肌动蛋白标记的CAFs表达。分析CAFs与NPC临床病理参数的关系。NPC组、鼻咽炎组及NPC转移组织中CAFs高密度表达分别为41.2%（35/85）、6.2%（2/32）和83.3%（10/12），三组间差异有统计学意义（<0.05）。卡方检验显示，CAFs密度与性别、年龄、N分期、治疗无显著相关性（>0.05），但与T分期及复发密切相关（<0.05）。Kaplan - Meier生存分析显示，高密度和低密度CAFs的平均总生存期分别为86.8个月和127.0个月。相应地，5年生存率分别为57.1%（20/35）和90.0%（45/50），两组间差异有统计学意义（<0.05）。Cox多因素分析显示，CAFs密度可作为NPC患者生存的独立预后因素（<0.05）。CAFs密度可能与NPC转移密切相关，也是NPC患者生存不良的有效预测因素。
