Tian Lu, Wang Xiaoyun, Wang Xiaoping, Lei Chaoliang, Zhu Fen
Hubei International Scientific and Technological Cooperation Base of Waste Conversion by Insects, Huazhong Agricultural University, Wuhan 430070, China; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
Gene. 2018 Feb 5;642:268-276. doi: 10.1016/j.gene.2017.11.041. Epub 2017 Nov 14.
In this study, starvation-, thermal- and heavy metal-associated expression of four small Musca domestica HSPs (abbreviated as MdomHSPs and they are MdomHSP10, MdomHSP27, MdomHSP27.1 and MdomHSP27.2) were determined. The following results were found: All MdomHSPs were significantly higher expressed during the active larval and adult stages than the egg and pupal stages; All MdomHSPs were expressed at relatively equal levels in the head, thorax and abdomen of adults; The expression of MdomHSP27 was significantly down-regulated in 4-day-old larvae that were starved for 6h, while the other 3 MdomHSPs were not significantly affected; Thermal treatment altered the expression of MdomHSPs in 4-day-old larvae: MdomHSP10 was significantly down-regulated in 4-day-old larvae that were maintained at 4°C and 37°C than in those that were maintained at 25°C; Lead, cadmium and chromium exposure influenced larval expression of MdomHSPs to varying degrees. The expression dynamic profile of MdomHSPs would contribute to the understanding of their physiological role in M. domestica.
在本研究中，测定了四种家蝇小热休克蛋白（简称为MdomHSPs，即MdomHSP10、MdomHSP27、MdomHSP27.1和MdomHSP27.2）在饥饿、热应激和重金属处理条件下的表达情况。结果如下：所有MdomHSPs在幼虫活跃期和成虫期的表达均显著高于卵期和蛹期；所有MdomHSPs在成虫的头部、胸部和腹部表达水平相对一致；饥饿6小时的4日龄幼虫中，MdomHSP27的表达显著下调，而其他3种MdomHSPs未受显著影响；热处理改变了4日龄幼虫中MdomHSPs的表达：与在25°C饲养的4日龄幼虫相比，在4°C和37°C饲养的4日龄幼虫中，MdomHSP10的表达显著下调；铅、镉和铬暴露对幼虫MdomHSPs的表达有不同程度的影响。MdomHSPs的表达动态谱将有助于理解它们在家蝇中的生理作用。
