神经降压素影响牛嗜铬细胞中阿片肽、儿茶酚胺和肌醇磷脂的代谢。

Neurotensin affects metabolism of opioid peptides, catecholamines and inositol phospholipids in bovine chromaffin cells.

作者信息

Bommer M, Herz A

机构信息

Department of Neuropharmacology, Max-Planck-Institut für Psychiatrie, Martinsried, Federal Republic of Germany.

出版信息

Life Sci. 1989;44(5):327-35. doi: 10.1016/0024-3205(89)90226-9.

DOI:10.1016/0024-3205(89)90226-9

PMID:2915604

Abstract

Bovine adrenal medullary cells released significant amounts of opioid pentapeptides Met- and Leu-enkephalin and catecholamines, when stimulated by neurotensin (NT). Maximal release induced by this peptide was about 40-50% of that seen after nicotinic activation of cholinergic receptors. Dose-response curves for neurotensin-induced secretion revealed an EC50 of 1x10(-6)M, thereby being in the range of that for acetylcholine or nicotine. Secretory effects were dependent on extracellular Ca++ and impaired by the Ca++ channel blocker D 600. Moreover NT produced an increase in opioid peptide cell content after 48 and 72 hrs of incubation. Besides affecting opioid peptide metabolism, NT significantly produced accumulation of inositol- 1-phosphates (IP1), the significance of which remains to be clarified in the observed metabolic effects.

摘要

当受到神经降压素（NT）刺激时，牛肾上腺髓质细胞会释放大量的阿片五肽甲硫氨酸脑啡肽和亮氨酸脑啡肽以及儿茶酚胺。该肽诱导的最大释放量约为烟碱激活胆碱能受体后释放量的40 - 50%。神经降压素诱导分泌的剂量反应曲线显示，其半数有效浓度（EC50）为1×10⁻⁶M，因此处于乙酰胆碱或尼古丁的浓度范围内。分泌效应依赖于细胞外钙离子，并且会被钙离子通道阻滞剂D 600削弱。此外，在孵育48小时和72小时后，神经降压素会使阿片肽细胞含量增加。除了影响阿片肽代谢外，神经降压素还显著导致肌醇-1-磷酸（IP1）的积累，其在观察到的代谢效应中的意义尚待阐明。