GFZF，一种与细胞周期调控和杂种不育有关的谷胱甘肽转移酶蛋白，是一种转录共激活因子。
GFZF, a Glutathione -Transferase Protein Implicated in Cell Cycle Regulation and Hybrid Inviability, Is a Transcriptional Coactivator.
机构信息
Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Department of Molecular and Medical Genetics, School of Medicine, OHSU Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA.
出版信息
Mol Cell Biol. 2018 Jan 29;38(4). doi: 10.1128/MCB.00476-17. Print 2018 Feb 15.
The core promoters of protein-encoding genes play a central role in regulating transcription. M1BP is a transcriptional activator that associates with a core promoter element known as Motif 1 that resides at thousands of genes in To gain insight into how M1BP functions, we identified an interacting protein called GFZF. GFZF had been previously identified in genetic screens for factors involved in maintenance of hybrid inviability, the G-M DNA damage checkpoint, and RAS/mitogen-activated protein kinase (MAPK) signaling, but its contribution to these processes was unknown. Here, we show that GFZF resides in the nucleus and functions as a transcriptional coactivator. In addition, we show that GFZF is a glutathione -transferase (GST). Thus, GFZF is the first transcriptional coactivator with intrinsic GST activity, and its identification as a transcriptional coactivator provides an explanation for its role in numerous biological processes.
蛋白质编码基因的核心启动子在调节转录中起着核心作用。M1BP 是一种转录激活因子，它与一个称为 Motif 1 的核心启动子元件结合，该元件存在于数千个基因中。为了深入了解 M1BP 的功能，我们鉴定了一种称为 GFZF 的相互作用蛋白。GFZF 先前在遗传筛选中被鉴定为参与维持杂种不育、G-M DNA 损伤检查点和 RAS/丝裂原激活蛋白激酶 (MAPK) 信号的因子，但它对这些过程的贡献尚不清楚。在这里，我们表明 GFZF 位于细胞核内，作为转录共激活因子发挥作用。此外，我们还表明，GFZF 是一种谷胱甘肽 -S- 转移酶 (GST)。因此，GFZF 是第一个具有内在 GST 活性的转录共激活因子，其作为转录共激活因子的鉴定为其在众多生物学过程中的作用提供了解释。
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