Ali I U, Merlo G, Callahan R, Lidereau R
Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, Maryland 20892.
Oncogene. 1989 Jan;4(1):89-92.
The int-2 gene, a member of the fibroblast growth factor (FGF) super-gene family, has previously been shown to be amplified in 16% of the 110 human breast tumors examined. In order to characterize the amplification unit containing the int-2 gene (chromosome 11q13), the same panel of breast tumors was screened for possible amplifications of other markers mapping between 11q11 and 11q24. Out of the eight additional genes analysed, simultaneous amplification of bcl-1 (11q13, a locus involved in hematopoietic malignancies) and hst (11q13, another member of the FGF family) was observed in 17/18 tumors with increased copy number of the int-2 gene. A single breast tumor showed amplification of int-2 oncogene only. Neither the bcl-1 nor the hst locus was individually amplified in any of the tumor DNAs examined.
int-2基因是成纤维细胞生长因子（FGF）超基因家族的成员，先前已发现在所检测的110例人类乳腺肿瘤中有16%存在该基因扩增。为了鉴定包含int-2基因（染色体11q13）的扩增单元，对同一组乳腺肿瘤进行筛查，以寻找定位于11q11和11q24之间的其他标记物是否可能发生扩增。在所分析的另外8个基因中，在18例int-2基因拷贝数增加的肿瘤中，有17例观察到bcl-1（11q13，一个与造血系统恶性肿瘤有关的位点）和hst（11q13，FGF家族的另一个成员）同时扩增。仅有1例乳腺肿瘤显示仅int-2癌基因发生扩增。在所检测的任何肿瘤DNA中，bcl-1和hst位点均未单独发生扩增。
