Tsuda T, Tahara E, Kajiyama G, Sakamoto H, Terada M, Sugimura T
Department of Pathology, Hiroshima University School of Medicine, Japan.
Cancer Res. 1989 Oct 15;49(20):5505-8.
We analyzed the alteration of the hst-1 and int-2 genes in 36 cases of esophageal squamous cell carcinoma, 42 cases of gastric adenocarcinoma, and 52 cases of colorectal adenocarcinoma. Coamplification of the hst-1 and int-2 genes was observed in 19 of 36 esophageal carcinomas (52%), 16 of 34 primary tumor tissues (47%), and 10 of 10 metastatic tumors (100%). The degree of amplification ranged from 4- to 8-fold. The incidence of hst-1 and int-2 gene coamplification was significantly higher in male patients than that in female patients (P less than 0.05). The coamplification of the hst-1 and int-2 genes had a tendency to correlate with clinical stage. The progesterone receptor gene, which is mapped to chromosome 11 at band q21-23, was not amplified in these esophageal carcinomas. Coamplification of the hst-1 and int-2 gene does not seem to imply increased numbers of chromosome 11, and the hst-1 and int-2 genes appear to be in same amplification unit on chromosome 11 at band q13. No coamplification of the hst-1 and int-2 genes was detected in gastric carcinomas and colorectal carcinomas. These results suggest that amplification of chromosomal locus of the hst-1 and int-2 genes might participate in carcinogenesis, in progression, and particularly in metastasis of esophageal carcinomas.
我们分析了36例食管鳞状细胞癌、42例胃腺癌和52例结肠腺癌中hst-1和int-2基因的改变。在36例食管癌中的19例（52%）、34例原发肿瘤组织中的16例（47%）以及10例转移瘤中的10例（100%）观察到hst-1和int-2基因的共扩增。扩增程度为4至8倍。男性患者中hst-1和int-2基因共扩增的发生率显著高于女性患者（P<0.05）。hst-1和int-2基因的共扩增有与临床分期相关的趋势。定位于11号染色体q21-23带的孕激素受体基因在这些食管癌中未扩增。hst-1和int-2基因的共扩增似乎并不意味着11号染色体数量增加，并且hst-1和int-2基因似乎在11号染色体q13带处于同一扩增单元。在胃癌和结肠癌中未检测到hst-1和int-2基因的共扩增。这些结果表明，hst-1和int-2基因染色体位点的扩增可能参与食管癌的致癌过程、进展，尤其是转移。
