Yoon Hye Young, Lee Si Young
a Department of Oral Microbiology, College of Dentistry, Research Institute of Oral Science , Gangneung-Wonju National University , Gangneung , Korea.
Biofouling. 2017 Nov;33(10):917-926. doi: 10.1080/08927014.2017.1391950. Epub 2017 Nov 21.
In this study, a laboratory model to reproduce dental unit waterline (DUWL) biofilms was developed using a CDC biofilm reactor (CBR). Bacteria obtained from DUWLs were filtered and cultured in Reasoner's 2A (R2A) for 10 days, and were subsequently stored at -70°C. This stock was cultivated on R2A in batch mode. After culturing for five days, the bacteria were inoculated into the CBR. Biofilms were grown on polyurethane tubing for four days. Biofilm accumulation and thickness was 1.3 × 10 CFU cm and 10-14 μm respectively, after four days. Bacteria in the biofilms included cocci and rods of short and medium lengths. In addition, 38 bacterial genera were detected in biofilms. In this study, the suitability and reproducibility of the CBR model for DUWL biofilm formation were demonstrated. The model provides a foundation for the development of bacterial control methods for DUWLs.
在本研究中，使用美国疾病控制与预防中心生物膜反应器（CBR）建立了一种用于重现牙科设备水线（DUWL）生物膜的实验室模型。从DUWLs获取的细菌经过过滤后，在Reasoner's 2A（R2A）培养基中培养10天，随后保存在-70°C。该菌种保藏物以分批模式在R2A培养基上培养。培养5天后，将细菌接种到CBR中。生物膜在聚氨酯管上生长4天。4天后，生物膜的累积量和厚度分别为1.3×10 CFU/cm和10 - 14μm。生物膜中的细菌包括短杆菌和中等长度的杆菌以及球菌。此外，在生物膜中检测到38个细菌属。在本研究中，证明了CBR模型对于DUWL生物膜形成的适用性和可重复性。该模型为开发DUWLs细菌控制方法奠定了基础。
