Roslyn J J, Abedin M Z, Strichartz S D, Abdou M S, Palant C E
Surgical, Medical, and Research Services, Sepulveda Veterans Administration Medical Center, Calif.
Surgery. 1989 Feb;105(2 Pt 1):207-12.
Recent studies indicate that biliary lipids influence in vivo gallbladder absorption and solute-coupled water flow. To clarify the electrophysiologic effects that underlie this phenomenon, prairie dog gallbladders were mounted in an Ussing-type chamber, and the influence of bile acids and varying ratios of bile acids and biliary phospholipids on transepithelial potential difference (Vms), resistance (Rt), and short-circuit current (Isc) was examined. Exposure to 5 mmol/L taurodeoxycholate (TDC) resulted in inhibition of Vms (p less than 0.01) and Isc (p less than 0.01) and an increase (p less than 0.05) in Rt. Subsequent perfusion with bile acids and phospholipids (5 mmol/L TDC + 0.3 mmol/L phosphatidylcholine [PC]) led to continued inhibition of ion transport. In contrast, exposure to 5 mmol/L TDC + 1.7 mmol/L PC resulted in a significant increase in transport, as manifested by an increase in Vms (p less than 0.02) and Isc (p less than 0.01) and a decrease in Rt (p less than 0.05) compared with bile acids. These results indicate that the ratio of phospholipids to bile salts modulates ion transport across prairie dog gallbladder and that this ratio may be an important determinant of gallbladder absorption in health and disease.
最近的研究表明，胆汁脂质影响体内胆囊的吸收以及溶质偶联的水流。为了阐明这一现象背后的电生理效应，将草原犬鼠的胆囊安装在Ussing型小室中，研究了胆汁酸以及不同比例的胆汁酸与胆汁磷脂对跨上皮电位差（Vms）、电阻（Rt）和短路电流（Isc）的影响。暴露于5 mmol/L牛磺脱氧胆酸盐（TDC）会导致Vms（p<0.01）和Isc（p<0.01）受到抑制，Rt增加（p<0.05）。随后用胆汁酸和磷脂（5 mmol/L TDC + 0.3 mmol/L磷脂酰胆碱[PC]）灌注会导致离子转运持续受到抑制。相比之下，暴露于5 mmol/L TDC + 1.7 mmol/L PC会导致转运显著增加，与胆汁酸相比，表现为Vms增加（p<0.02）和Isc增加（p<0.01）以及Rt降低（p<0.05）。这些结果表明，磷脂与胆盐的比例调节草原犬鼠胆囊的离子转运，并且该比例可能是健康和疾病状态下胆囊吸收的重要决定因素。
