Singh Chandu, K V Sripathy, S P Jeevan Kumar, K Bhojaraja Naik, Pal Govind, K Udaya Bhaskar, K V Ramesh, G Somasundaram
ICAR- Indian Institute of Seed Science, Mau, Uttar Pradesh, 275103, India.
Seed Production Unit, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Rice (N Y). 2017 Nov 21;10(1):48. doi: 10.1186/s12284-017-0187-9.
Rice aleurone layer develops different colours with various chemical tests that may help to develop some rapid tests for identification/grouping of rice varieties. Understanding the colour inheritance pattern could enable to develop chemical clues that may help for genetic purity analysis along with grow-out-test.
In this study, inheritance pattern of aleurone layer colour was studied in parents, F and F progenies derived from the crosses IR 36 × Acc. No. 2693 and IR 64 × Acc. No. 2693. The parent IR 36 showed light yellow (NaOH/KOH) and brown (phenol/modified phenol test) colour; whereas, Acc. No. 2693 revealed wine red/dark wine red (NaOH/KOH) and light brown colour/no reaction (phenol/modified phenol test). In contrary, another parent IR 64 exhibited light yellow (KOH/NaOH) and dark brown (phenol, modified phenol) colour. Both the F showed an intermediate light wine red colour (NaOH/KOH) and dark brown (phenol and modified phenol) colour, which is dominant over their one of the parents. The colour pattern with standard phenol/modified phenol, NaOH and KOH tests in F progenies of both the crosses showed 9:7 (complementary gene interaction) and 11:5 ratios (reciprocal dominance modification of recessive alleles), respectively.
Our findings clearly elucidate the colour inheritance pattern in rice that may facilitate to develop rapid chemical tests to identify/ group the varieties for genetic purity analysis.
水稻糊粉层在各种化学测试中会呈现不同颜色,这可能有助于开发一些快速测试方法来鉴定/区分水稻品种。了解颜色遗传模式有助于找到化学线索,从而与种植鉴定试验一起用于遗传纯度分析。
在本研究中,对杂交组合IR 36×编号2693和IR 64×编号2693的亲本、F₁和F₂后代的糊粉层颜色遗传模式进行了研究。亲本IR 36在氢氧化钠/氢氧化钾测试中呈浅黄色,在苯酚/改良苯酚测试中呈棕色;而编号2693在氢氧化钠/氢氧化钾测试中呈现酒红色/深酒红色,在苯酚/改良苯酚测试中呈浅棕色/无反应。相反,另一个亲本IR 64在氢氧化钾/氢氧化钠测试中呈浅黄色,在苯酚、改良苯酚测试中呈深棕色。两个F₁均呈现中间型的浅酒红色(氢氧化钠/氢氧化钾测试)和深棕色(苯酚和改良苯酚测试),其颜色比亲本之一更占优势。两个杂交组合的F₂后代在标准苯酚/改良苯酚、氢氧化钠和氢氧化钾测试中的颜色模式分别显示出9:7(互补基因相互作用)和11:5的比例(隐性等位基因的互作显性修饰)。
我们的研究结果清楚地阐明了水稻的颜色遗传模式,这可能有助于开发快速化学测试方法来鉴定/区分品种以进行遗传纯度分析。