Socha W, Rola J, Urban-Chmiel R, Żmudziński J F
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Pol J Vet Sci. 2017 Sep 26;20(3):619-622. doi: 10.1515/pjvs-2017-0078.
Bovine herpesvirus-1 (BoHV-1), a causative agent of Infectious Bovine Rhinotracheitis (IBR), is responsible for high economic losses in cattle farming industry. The use of testing methods that allow early detection of BoHV-1-infected animals is a key element of each program of IBR eradication. The aim of the study was to design and evaluate two variants of LAMP isothermal tests with SYBR Green fluorescence probes, specific to the genes encoding gD and gE glycoproteins of BoHV-1. LAMP gE BoHV-1 assay was able to distinguish between gE- and gE+ strains of the virus. Both LAMP gD and gE assays were specific to BoHV-1 and did not react with other related to BoHV-1 alphaherpesviruses. Sensitivity of LAMP gD was 2x104 copies of the viral genome whereas for LAMP gE it was 2x105. Diagnostic sensitivity calculated for LAMP gD was 64.7% whereas for LAMP gE it was 80%. Diagnostic specificity for LAMP gD and LAMP gE was 78.9% and 89.3%, respectively. LAMP assay can be a rapid and simple method of diagnosis of acute BoHV-1 infections and discrimination of gE- strains. However, relatively low diagnostic sensitivity of the method can limit its use in routine diagnostics.
牛疱疹病毒1型（BoHV-1）是传染性牛鼻气管炎（IBR）的病原体，给养牛业造成了巨大的经济损失。采用能够早期检测出感染BoHV-1动物的检测方法是IBR根除计划的关键要素。本研究的目的是设计并评估两种带有SYBR Green荧光探针的环介导等温扩增（LAMP）检测方法的变体，它们针对BoHV-1编码gD和gE糖蛋白的基因具有特异性。LAMP gE BoHV-1检测方法能够区分该病毒的gE-和gE+毒株。LAMP gD和gE检测方法均对BoHV-1具有特异性，且不与其他与BoHV-1相关的甲型疱疹病毒发生反应。LAMP gD的灵敏度为病毒基因组的2×104个拷贝，而LAMP gE的灵敏度为2×105个拷贝。LAMP gD计算得出的诊断灵敏度为64.7%，而LAMP gE为80%。LAMP gD和LAMP gE的诊断特异性分别为78.9%和89.3%。LAMP检测方法可以成为诊断急性BoHV-1感染和区分gE-毒株的快速简便方法。然而，该方法相对较低的诊断灵敏度可能会限制其在常规诊断中的应用。
