School of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Islamic Republic of Iran.
Experimental Physics, Materials Physics, Materials Science, University of Tabriz, Tabriz, Islamic Republic of Iran.
Biomed Pharmacother. 2017 Dec;96:1335-1340. doi: 10.1016/j.biopha.2017.11.077. Epub 2017 Nov 21.
The objective of this study was to investigate the effects of separate and concurrent supplementation of natural nano-sized clinoptilolite (NCLN) and Nigella sativa (NS) on oxidative stress (OS), anti-oxidative parameters and body weight (BW) in high-fat-diet (HFD)/streptozotocin (STZ)-induced diabetic rats.
In this experimental study, 42 male Wistar rats were divided into diabetic (n=36) and non-diabetic (n=6) groups. The diabetic group (DG) was fed with a HFD for one month, then injected with intra-peritoneal single dose STZ (35 mg/kg BW). The DG was divided into 4 subgroups: [1] control (DC), [2] NS 1%/food, [3] NCLN 2%/food, [4] NS 1%/food + NCLN 2%/food. At the end of the 7th week, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPX) levels and total antioxidant capacity (TAC) were measured.
The MDA level was decreased in the NCLN (p = 0.011) and NCLN+NS (p = 0.007) groups compared to the DC group. The GPX level increased in the NS and NCLN groups compared to the DC group (p = 0.014 and p = 0.034). In addition, the level of TAC demonstrated increase in the untreated DG and NS groups, as compared to the normal control (NC) group (p = 0.031 and p = 0.024). Moreover, in the NS+NCLN group, the level of SOD decreased in comparison to the NS and NCLN groups (p < 0.01). At the end of the 7th week, BW decreased in the diabetic subgroups in comparison to the NC group. Treatment with NS and/or NS+NCLN insignificantly prevented severe weight loss in the fifth week of the treatment.
According to results, separate supplementation of NS and NCLN was more beneficent on anti-oxidative parameters than concurrent supplementation of NS and NCLN.
本研究旨在探讨分别补充天然纳米沸石（NCLN）和黑种草（NS）以及同时补充这两种物质对高脂肪饮食（HFD）/链脲佐菌素（STZ）诱导的糖尿病大鼠氧化应激（OS）、抗氧化参数和体重（BW）的影响。
在这项实验研究中，将 42 只雄性 Wistar 大鼠分为糖尿病（n=36）和非糖尿病（n=6）两组。糖尿病组（DG）先喂养 HFD 一个月，然后腹腔内单次注射 STZ（35mg/kg BW）。DG 再分为 4 个亚组：[1]对照组（DC），[2]NS1%/食物，[3]NCLN2%/食物，[4]NS1%/食物+NCLN2%/食物。在第 7 周结束时，测量丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）水平和总抗氧化能力（TAC）。
与 DC 组相比，NCLN（p=0.011）和 NCLN+NS（p=0.007）组的 MDA 水平降低。与 DC 组相比，NS 和 NCLN 组的 GPX 水平升高（p=0.014 和 p=0.034）。此外，与正常对照组（NC）相比，未经处理的 DG 和 NS 组的 TAC 水平升高（p=0.031 和 p=0.024）。此外，在 NS+NCLN 组中，SOD 水平与 NS 和 NCLN 组相比下降（p<0.01）。第 7 周结束时，与 NC 组相比，糖尿病亚组的 BW 下降。NS 和/或 NS+NCLN 的治疗并不能显著防止治疗第 5 周时的严重体重减轻。
根据结果，与同时补充 NS 和 NCLN 相比，分别补充 NS 和 NCLN 对抗氧化参数更有益。
