Bisby R H, Birch D J
Department of Biological Sciences, University of Salford, UK.
Biochem Biophys Res Commun. 1989 Jan 31;158(2):386-91. doi: 10.1016/s0006-291x(89)80059-2.
Rotational mobility in fluid phase dipalmitoylphosphatidylcholine unilamellar vesicles containing alpha-tocopherol has been studied by time-resolved anisotropy measurements of fluorescence from a diphenylhexatriene-phosphatidylcholine conjugate. The results are analysed using a simple wobbling-in-cone model. The diphenylhexatriene probe shows an increasing order parameter and more restricted wobbling with increasing alpha-tocopherol content of the membrane. The diffusional rate for wobbling was found not to change significantly.
通过对二苯基己三烯 - 磷脂酰胆碱共轭物荧光的时间分辨各向异性测量，研究了含有α - 生育酚的液相二棕榈酰磷脂酰胆碱单层囊泡中的旋转流动性。使用简单的锥体内摆动模型对结果进行分析。随着膜中α - 生育酚含量的增加，二苯基己三烯探针显示出增加的序参数和更受限的摆动。发现摆动的扩散速率没有显著变化。
