Dunlap Christopher A, Ramirez José Luis, Mascarin Gabriel Moura, Labeda David P
Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA.
Embrapa Meio Ambiente, Rodovia SP-340, km 127.5, S/N - Tanquinho Velho, Jaguariúna, SP, 13820-000, Brazil.
Antonie Van Leeuwenhoek. 2018 Jun;111(6):897-904. doi: 10.1007/s10482-017-0988-2. Epub 2017 Nov 23.
We report the development of a publicly accessible, curated nucleotide sequence database of hypocrealean entomopathogenic fungi. The goal is to provide a platform for users to easily access sequence data from taxonomic reference strains. The database can be used to accurately identify unknown entomopathogenic fungi based on sequence data for a variety of phylogenetically informative loci. The database provides full multi-locus sequence alignment capabilities. The initial release contains data compiled for 525 strains covering the phylogenetic diversity of three important entomopathogenic families: Clavicipitaceae, Cordycipitaceae, and Ophiocordycipitaceae. Furthermore, Entomopathogen ID can be expanded to other fungal clades of insect pathogens, as sequence data becomes available. The database will allow isolate characterisation and evolutionary analyses. We contend that this freely available, web-accessible database will facilitate the broader community to accurately identify fungal entomopathogens, which will allow users to communicate research results more effectively.
我们报告了一个可公开访问的、经过整理的肉座菌目昆虫病原真菌核苷酸序列数据库的开发情况。目标是为用户提供一个平台,以便轻松访问分类参考菌株的序列数据。该数据库可用于根据各种系统发育信息位点的序列数据准确鉴定未知的昆虫病原真菌。该数据库提供完整的多位点序列比对功能。初始版本包含为525个菌株汇编的数据,涵盖了三个重要昆虫病原科的系统发育多样性:麦角菌科、虫草菌科和蛇形虫草菌科。此外,随着序列数据的获得,昆虫病原体识别数据库可以扩展到其他昆虫病原真菌类群。该数据库将允许进行分离株特征分析和进化分析。我们认为,这个免费提供、可通过网络访问的数据库将有助于更广泛的群体准确鉴定真菌昆虫病原体,这将使用户能够更有效地交流研究结果。