Rotolo Maria C, Klein Julia, Pacifici Roberta, Busardo Francesco Paolo, Pichini Simona, Marchei Emilia
National Centre on Drug Addiction and Doping, Istituto Superiore di Sanita, Rome, Italy.
Drug Testing Consultants, 265 Russell Hill Road #801, Toronto, M4V2T3, Canada.
Curr Pharm Biotechnol. 2017;18(10):834-839. doi: 10.2174/1389201018666171122114526.
New psychoactive substances (NPS) are a heterogenic group of substances with different chemical structures and psychotropic effects.
Many pharmacotoxicological laboratories performing drug testing in conventional and nonconventional biological matrices for clinical and forensic purposes do not include screening procedures for NPS in their routine protocols. This is mainly due to the continued entry in the market of newly synthesized products, the low availability of reference standards, in particular of their metabolites, the low availability of immunochemical kits, etc. Moreover, many of the new compounds are very potent, and low doses ingested will lead to low concentrations in biological matrices, especially in hair.
Hair analysis has become a powerful tool for detecting chronic drug use and has become a routine technique in forensic toxicology laboratories.
The aim of this study was to set up analytical strategies to identify repeated consumption of NPS by hair analysis. Although UHPLC-MS/MS may represent the elective technique in studying NPS, a combination of both GC-MS and UHPLC-MS/MS techniques is useful in creating a complete toxicological image.
新型精神活性物质（NPS）是一类具有不同化学结构和精神活性作用的异质性物质。
许多为临床和法医目的在常规和非常规生物基质中进行药物检测的药物毒理学实验室，在其常规方案中未包括NPS的筛查程序。这主要是由于新合成产品不断进入市场、参考标准品尤其是其代谢物的可得性低、免疫化学试剂盒的可得性低等原因。此外，许多新化合物效力很强，摄入低剂量会导致生物基质中浓度很低，尤其是在毛发中。
毛发分析已成为检测长期药物使用的有力工具，并已成为法医毒理学实验室的常规技术。
本研究的目的是建立分析策略，通过毛发分析识别NPS的反复使用情况。尽管超高效液相色谱-串联质谱（UHPLC-MS/MS）可能是研究NPS的首选技术，但气相色谱-质谱（GC-MS）和UHPLC-MS/MS技术相结合有助于形成完整的毒理学图像。
