Schneeberger A L, Thompson R T, Driedger A A, Finley R J, Inculet R I
Department of Surgery, Victoria Hospital, University of Western Ontario, London, Canada.
Cancer Res. 1989 Mar 1;49(5):1160-4.
The effect of increasing tumor burden on host liver and skeletal muscle energy status was studied using P-31 nuclear magnetic resonance spectroscopy (NMR), in rats inoculated with a nonmetastasizing methylcholanthrene-induced sarcoma (TB), and compared to nontumor bearing (NTB) and pair-fed (PF) rats. During the 28-day study, serial measurements of body weight, food intake, and tumor volume were obtained. Using a 0.9-cm double-turn surface coil, weekly NMR measurements were obtained from liver and skeletal muscle. An increasing ratio of [Pi]/[ATP] was used as one measure of intracellular energy depletion. [Pi]/[ATP] in NTB rats remained constant over time at 0.78 +/- 0.10 in liver, and 0.30 +/- 0.10 in skeletal muscle. In TB rats, the [Pi]/[ATP] ratio increased significantly in liver (P = 0.00002) and skeletal muscle (P = 0.04) with increasing tumor burden. In PF rats, no significant change occurred in [Pi]/[ATP] in liver or skeletal muscle, indicating that declining food intake was not responsible for the change in [Pi]/[ATP] seen in TB rats. Surface-coil spectroscopy of liver and skeletal muscle permits serial measurement of visceral energy stores. Increasing tumor burden results in early, ongoing depletion of energy stores as reflected by increasing [Pi]/[ATP] in these organs.
利用磷-31核磁共振波谱法（NMR），研究了接种非转移性甲基胆蒽诱导肉瘤（TB）的大鼠中肿瘤负荷增加对宿主肝脏和骨骼肌能量状态的影响，并与未患肿瘤（NTB）和配对饲养（PF）的大鼠进行了比较。在为期28天的研究中，连续测量了体重、食物摄入量和肿瘤体积。使用一个0.9厘米的双匝表面线圈，每周从肝脏和骨骼肌获取NMR测量值。[Pi]/[ATP]比值增加被用作细胞内能量耗竭的一种指标。NTB大鼠肝脏中的[Pi]/[ATP]随时间保持恒定，为0.78±0.10，骨骼肌中为0.30±0.10。在TB大鼠中，随着肿瘤负荷增加，肝脏（P = 0.00002）和骨骼肌（P = 0.04）中的[Pi]/[ATP]比值显著增加。在PF大鼠中，肝脏或骨骼肌中的[Pi]/[ATP]没有显著变化，这表明食物摄入量下降不是TB大鼠中[Pi]/[ATP]变化的原因。肝脏和骨骼肌的表面线圈波谱法允许对内脏能量储备进行连续测量。肿瘤负荷增加导致能量储备早期持续耗竭，这在这些器官中表现为[Pi]/[ATP]增加。
