College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Bioresour Technol. 2018 Feb;250:204-213. doi: 10.1016/j.biortech.2017.09.160. Epub 2017 Oct 31.
In order to improve the methane yield, the alkaline and biological pretreatments on anaerobic digestion (AD) were investigated. Three treatments were tested: NaOH, biological (enzyme and fungi), and combined NaOH with biological. The maximum reducing sugar concentrations were obtained using Enzyme T (2.20 mg/mL) on the 6th day. The methane yield of NaOH + Enzyme A was 300.85 mL/g TS, 20.24% higher than the control. Methane yield obtained from Enzyme (T + A) and Enzyme T pretreatments were 277.03 and 273.75 mL/g TS, respectively, which were as effective as 1% NaOH (276.16 mL/g TS) in boosting methane production, and are environmentally friendly and inexpensive biological substitutes. Fungal pretreatment inhibited methane fermentation of maize straw, 15.68% was reduced by T + A compared with the control. The simultaneous reduction of DM, cellulose and hemicellulose achieved high methane yields. This study provides important guidance for the application of enzymes to AD from lignocellulosic agricultural waste.
为了提高甲烷产量，研究了厌氧消化（AD）的碱性和生物预处理。测试了三种处理方法：NaOH、生物（酶和真菌）和 NaOH 与生物联合。第 6 天，酶 T（2.20mg/mL）获得的最大还原糖浓度最高。NaOH+酶 A 的甲烷产量为 300.85mL/g TS，比对照提高了 20.24%。酶（T+A）和酶 T 预处理的甲烷产量分别为 277.03 和 273.75mL/g TS，与 1%NaOH（276.16mL/g TS）一样有效，可促进甲烷生成，且环保且廉价的生物替代品。真菌预处理抑制了玉米秸秆的甲烷发酵，与对照相比，T+A 减少了 15.68%。DM、纤维素和半纤维素的同时减少实现了高甲烷产量。本研究为酶在木质纤维素农业废弃物 AD 中的应用提供了重要指导。
