Robertson Lesley A
Department of Biotechnology and Delft Science Centre, Delft University of Technology, Mijnbouwstraat 120, 2628RX, Delft, The Netherlands.
FEMS Microbiol Lett. 2017 Dec 29;364(24). doi: 10.1093/femsle/fnx247.
Possible techniques for lighting opaque samples while using Van Leeuwenhoek microscopes have been tested, and the results are presented in relation to published material. The design of the microscope causes the sample to be in shadow with any form of top lighting. It is therefore suggested that Van Leeuwenhoek's hinted 'particular method of observing' might refer to a different style of microscope as shown in the frontispiece of the sale catalogue for his microscopes, and available at that time for purchase from sellers of optical equipment.
使用范·列文虎克显微镜时照亮不透明样本的可能技术已经过测试,并根据已发表的材料呈现了结果。显微镜的设计使得样本在任何形式的顶部照明下都处于阴影中。因此,有人认为范·列文虎克所暗示的“特殊观察方法”可能指的是一种不同样式的显微镜,如他的显微镜销售目录卷首插图中所示,并且当时可以从光学设备销售商处购买到。