Husain Fasahath, Nikaido Hiroshi
Doctor Evidence, LLC, 301 Arizona Avenue, Suite 301, Santa Monica, CA, 90401, USA.
Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Methods Mol Biol. 2018;1700:167-175. doi: 10.1007/978-1-4939-7454-2_10.
An experimental approach to detect the path a substrate takes through a complex membrane protein is described with emphasis on technical approach and theoretical considerations. The protocols for bacterial culture preparation, membrane protein purification, fluorescent assay standardization, data collection, and data analysis are provided. Useful software tools are recommended.
本文描述了一种检测底物通过复杂膜蛋白所走路径的实验方法,重点在于技术方法和理论考量。文中提供了细菌培养制备、膜蛋白纯化、荧光测定标准化、数据收集及数据分析的方案。还推荐了实用的软件工具。