Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3202, USA.
J Bacteriol. 2011 Oct;193(20):5847-9. doi: 10.1128/JB.05759-11. Epub 2011 Aug 19.
The path of substrates in the multidrug efflux pump AcrB of Escherichia coli was examined by using labeling with a lipophilic substrate mimic, Bodipy FL maleimide. Four (out of eight) residues in the vestibule bound the dye, suggesting its role in substrate transport, whereas only one (out of nine) residue in the central cavity tested positive.
通过使用脂溶性底物类似物 Bodipy FL 马来酰亚胺进行标记，研究了大肠杆菌多药外排泵 AcrB 中的底物途径。在入口处有四个（八个中的四个）残基结合了染料，表明其在底物转运中的作用，而在中央腔中只有一个（九个中的一个）残基呈阳性。
