Sanes D H, Merickel M, Rubel E W
Department of Otolaryngology, University of Virginia Medical Center, Charlottesville 22908.
J Comp Neurol. 1989 Jan 15;279(3):436-44. doi: 10.1002/cne.902790308.
We have investigated developmental alterations in the tonotopic projection of the gerbil lateral superior olive. Single neurons were characterized in the frequency domain and the recording site marked with fast green. Transverse tissue sections from the auditory brainstem of each animal were visualized with a video-equipped microscope, and the image was digitized for subsequent alignment. The three-dimensional display indicated little variation in the rostrocaudal axis, allowing us to collapse the data into a two-dimensional tonotopic map. The tonotopic map was found to change with age such that the characteristic frequency of neurons in a given anatomical location became successively higher during development. These results are consistent with the hypothesis that the place code gradually shifts in the developing cochlea.
我们研究了沙鼠外侧上橄榄核音频定位投射的发育变化。在频域中对单个神经元进行特征分析，并用固绿标记记录位点。用配备视频的显微镜观察每只动物听觉脑干的横向组织切片，并将图像数字化以便后续对齐。三维显示表明在前后轴上变化很小，这使我们能够将数据整合为二维音频定位图。发现音频定位图随年龄变化，以至于在发育过程中，给定解剖位置的神经元特征频率逐渐升高。这些结果与发育中的耳蜗中位置编码逐渐变化的假设一致。
