在A549细胞系中，乙醛脱氢酶（ALDH）的活性受Nodal和组胺的调节。
ALDH enzyme activity is regulated by Nodal and histamine in the A549 cell line.
作者信息
Jiang Yang, Li Hui, Wang Yi, Tian Tian, He Yan, Jin Yinji, Han Changsong, Jin Xiaoming, Zhang Fengmin, Morii Eiichi
机构信息
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
Department of Gastroenterology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
出版信息
Oncol Lett. 2017 Dec;14(6):6955-6961. doi: 10.3892/ol.2017.7057. Epub 2017 Sep 25.
The present study aimed to examine whether the enzyme activity of aldehyde dehydrogenase (ALDH) was regulated by Nodal and histamine in the human alveolar adenocarcinoma A549 cell line. The regulated enzyme activity of ALDH was analyzed by flow cytometry in the A549 cell line. ALDH1 and Nodal expression was investigated by immunohistochemistry in28 cases of lung mixed adenocarcinoma. The enzyme activity of ALDH was upregulated by histamine and agonists of histamine H1 receptor (H1R) and histamine H2 receptor (H2R). ALDH activity was also downregulated by recombinant human Nodal and antagonists of H1R and H2R in the A549 cell line. In addition, expression of Nodal and ALDH1 were inversely correlated in lung mixed adenocarcinoma. ALDH enzyme activity was regulated by Nodal and histamine in lung adenocarcinoma.
本研究旨在检测在人肺泡腺癌A549细胞系中，醛脱氢酶（ALDH）的酶活性是否受Nodal和组胺调控。通过流式细胞术分析A549细胞系中ALDH的调控酶活性。采用免疫组织化学法研究28例肺混合腺癌中ALDH1和Nodal的表达情况。组胺以及组胺H1受体（H1R）和组胺H2受体（H2R）激动剂可上调A549细胞系中ALDH的酶活性。重组人Nodal以及H1R和H2R拮抗剂可下调A549细胞系中ALDH的活性。此外，在肺混合腺癌中，Nodal和ALDH1的表达呈负相关。在肺腺癌中，ALDH酶活性受Nodal和组胺调控。
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