Swartzendruber D C, Wertz P W, Kitko D J, Madison K C, Downing D T
Marshall Dermatology Research Laboratories, Department of Dermatology, University of Iowa College of Medicine, Iowa City 52242.
J Invest Dermatol. 1989 Feb;92(2):251-7. doi: 10.1111/1523-1747.ep12276794.
Intercellular lipid lamellae in the stratum corneum constitute the barrier to water diffusion and may also play a role in cohesion between corneocytes. The lamellae arise from stacks of lamellar disks that are extruded from the granular cells and then fuse edge-to-edge to form sheets. It has been proposed that each lamellar disk is formed from a flattened vesicle, and therefore consists of two lipid bilayers in close apposition. In the present study, electron microscopic examination of ruthenium-tetroxide-fixed stratum corneum from mouse, pig, and human skin revealed that the double bilayer pattern persists in the intercellular lamellae. In addition, distinctive patterning of the intercellular lamellae has led us to propose novel molecular arrangements of the intercellular lipids. These include interlamellar sharing of lipid chains to produce lipid monolayers between pairs of bilayers. The pattern reflects the provenance of the intercellular lamellae from lamellar granule disks and the nonrandom orientation of the lamellar lipids.
角质层中的细胞间脂质薄片构成了水扩散的屏障，并且可能在角质形成细胞之间的黏附中也发挥作用。这些薄片源自从颗粒细胞中挤出的层状盘堆叠，然后边缘对边缘融合形成薄片。有人提出每个层状盘由一个扁平囊泡形成，因此由两个紧密相邻的脂质双层组成。在本研究中，对来自小鼠、猪和人类皮肤的四氧化钌固定角质层进行电子显微镜检查发现，双双层模式在细胞间薄片中持续存在。此外，细胞间薄片的独特图案使我们提出了细胞间脂质的新分子排列方式。这些包括脂质链的层间共享，以在双层对之间产生脂质单层。这种模式反映了细胞间薄片源自层状颗粒盘以及层状脂质的非随机取向。
