Clerkin Kevin J, See Sarah B, Farr Maryjane A, Restaino Susan W, Serban Geo, Latif Farhana, Li Lingzhi, Colombo Paolo C, Vlad George, Ray Bryan, Vasilescu Elena R, Zorn Emmanuel
Division of Cardiology, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, NY.
Columbia Center for Translational Immunology, Columbia University College of Physicians and Surgeons, New York, NY.
Transplant Direct. 2017 Oct 2;3(11):e218. doi: 10.1097/TXD.0000000000000734. eCollection 2017 Nov.
Allospecific anti-HLA antibodies (Abs) are associated with rejection of solid organ grafts. The 2 main kits to detect anti-HLA Ab in patient serum are commercialized by Immucor and One Lambda/ThermoFisher. We sought to compare the performance of both platforms.
Background-adjusted mean fluorescence intensity (MFI) values were used from both platforms to compare sera collected from 125 pretransplant and posttransplant heart and lung transplant recipients.
Most HLA class I (94.5%) and HLA class II (89%) Abs with moderate to high MFI titer (≥4000) were detected by both assays. A modest correlation was observed between MFI values obtained from the 2 assays for both class I ( = 0.3, = 0.09, < 0.0001) and class II Ab ( = 0.707, = 0.5, < 0.0001). Both assays detected anti-class I and II Ab that the other did not; however, no specific HLA allele was detected preferentially by either of the 2 assays. For a limited number of discrepant sera, dilution resulted in comparable reactivity profiles between the 2 platforms.
Immucor and One Lambda/ThermoFisher assays have a similar, albeit nonidentical, ability to detect anti-HLA Ab. Although the correlation between the assays was present, significant variances exist, some of which can be explained by a dilution-sensitive "prozone" effect.
同种特异性抗人白细胞抗原（HLA）抗体与实体器官移植排斥反应相关。检测患者血清中抗HLA抗体的2种主要试剂盒由Immucor公司和One Lambda/ThermoFisher公司商业化生产。我们试图比较这两种平台的性能。
使用两种平台的背景校正平均荧光强度（MFI）值来比较从125例心脏和肺移植术前及术后受者采集的血清。
两种检测方法均可检测到大多数MFI滴度中等至高（≥4000）的HLAⅠ类（94.5%）和HLAⅡ类（89%）抗体。对于Ⅰ类抗体（r = 0.3，P = 0.09，P < 0.0001）和Ⅱ类抗体（r = 0.707，P = 0.5，P < 0.0001），两种检测方法获得的MFI值之间均观察到适度的相关性。两种检测方法均检测到对方未检测到的抗Ⅰ类和Ⅱ类抗体；然而，两种检测方法均未优先检测到特定的HLA等位基因。对于数量有限的不一致血清，稀释后两种平台之间的反应性谱具有可比性。
Immucor和One Lambda/ThermoFisher检测方法检测抗HLA抗体的能力相似，但不完全相同。虽然两种检测方法之间存在相关性，但仍存在显著差异，其中一些差异可由稀释敏感的“前带”效应解释。
