Fredriksson Nils Johan, Hermansson Malte, Wilén Britt-Marie
a Department of Architecture and Civil Engineering, Water Environment Technology , Chalmers University of Technology , Gothenburg , Sweden.
b Department of Chemistry and Molecular Biology, Microbiology , University of Gothenburg , Gothenburg , Sweden.
Environ Technol. 2019 Mar;40(7):912-928. doi: 10.1080/09593330.2017.1411396. Epub 2017 Dec 7.
The operational efficiency of activated sludge wastewater treatment plants depends to a large extent on the microbial community structure of the activated sludge. The aims of this paper are to describe the composition of the bacterial community in a Swedish full-scale activated sludge wastewater treatment plant, to describe the dynamics of the community and to elucidate possible causes for bacterial community composition changes. The bacterial community composition in the activated sludge was described using 16S rRNA gene libraries and monitored for 15 months by a terminal restriction fragment (T-RF) length polymorphism (T-RFLP) analysis of the 16S rRNA gene. Despite variable environmental conditions, a large fraction of the observed T-RFs were present at all times, making up at least 50% in all samples, possibly representing a relatively stable core fraction of the bacterial community. However, the proportions of the different T-RFs in this fraction as well as the T-RFs in the more variable fraction showed a significant variation over time and temperature. The difference in community composition between summer and winter coincided with observed differences in floc structure. These observations suggest a relationship between floc properties and bacterial community composition, although additional experiments are required to determine causality.
活性污泥法污水处理厂的运行效率在很大程度上取决于活性污泥的微生物群落结构。本文旨在描述瑞典一座全尺寸活性污泥法污水处理厂中细菌群落的组成，描述群落的动态变化，并阐明细菌群落组成变化的可能原因。利用16S rRNA基因文库描述活性污泥中的细菌群落组成，并通过对16S rRNA基因进行末端限制性片段（T-RF）长度多态性（T-RFLP）分析，对其进行了15个月的监测。尽管环境条件多变，但大部分观察到的T-RF在所有时间都存在，在所有样本中至少占50%，可能代表了细菌群落中相对稳定的核心部分。然而，这一部分中不同T-RF的比例以及变化较大部分中的T-RF比例随时间和温度呈现出显著变化。夏季和冬季群落组成的差异与观察到的絮体结构差异一致。这些观察结果表明絮体性质与细菌群落组成之间存在关联，尽管还需要额外的实验来确定因果关系。
