Kuittinen Tea, Rovio Päivi, Staff Synnöve, Luukkaala Tiina, Kallioniemi Anne, Grénman Seija, Laurila Marita, Mäenpää Johanna
Department of Obstetrics and Gynaecology, Tampere University Hospital, Tampere, Finland
Department of Obstetrics and Gynaecology, Tampere University Hospital, Tampere, Finland.
Anticancer Res. 2017 Dec;37(12):6575-6581. doi: 10.21873/anticanres.12114.
BACKGROUND/AIM: Endometrial cancer cells are known to be sensitive to carboplatin and paclitaxel. Furthermore, vitamin D (1,25-D3) has been reported to inhibit endometrial cancer cell growth both as a single agent and combined with carboplatin. However, there are no studies comparing the effect of paclitaxel and carboplatin as single agents vs. in combination in endometrial cancer cell lines. Neither has the effect of 1,25-D3 been studied with paclitaxel. The present study investigated the effect of paclitaxel, carboplatin and 1,25-D3 on the growth of endometrial cancer cells in vitro.
Two endometrial adenocarcinoma cell lines (UT-EC-1 and UT-EC-3) were cultured with different doses of paclitaxel, carboplatin and 1,25-D3. The cellular VDR (vitamin D receptor) mRNA levels were measured and the expression of estrogen (ER) and progesterone (PR) receptors by the cells was determined.
In the UT-EC-1 cell line the growth inhibition was 72% with paclitaxel, 54% with carboplatin and 73% with the combination of these compounds. The corresponding numbers in UT-EC-3 were 70%, 33% and 65%, respectively. 1,25-D3 suppressed cell growth 88% with paclitaxel, 63% with carboplatin and 87% with their combination in the UT-EC-1 cell line.
In both cell lines, single-agent paclitaxel was as effective as the combination of the compounds and more effective than single carboplatin. 1,25-D3 may further contribute to the cytotoxic effect of these agents.
背景/目的：已知子宫内膜癌细胞对卡铂和紫杉醇敏感。此外，据报道维生素D（1,25 - D3）无论是作为单一药物还是与卡铂联合使用，均可抑制子宫内膜癌细胞生长。然而，尚无研究比较紫杉醇和卡铂单药与联合用药对子宫内膜癌细胞系的影响。也未研究1,25 - D3与紫杉醇联合使用的效果。本研究调查了紫杉醇、卡铂和1,25 - D3对体外子宫内膜癌细胞生长的影响。
用不同剂量的紫杉醇、卡铂和1,25 - D3培养两种子宫内膜腺癌细胞系（UT - EC - 1和UT - EC - 3）。检测细胞维生素D受体（VDR）mRNA水平，并测定细胞雌激素（ER）和孕激素（PR）受体的表达。
在UT - EC - 1细胞系中，紫杉醇的生长抑制率为72%，卡铂为54%，二者联合为73%。UT - EC - 3细胞系中的相应数值分别为70%、33%和65%。在UT - EC - 1细胞系中，1,25 - D3与紫杉醇联合时细胞生长抑制率为88%，与卡铂联合时为63%，二者联合时为87%。
在两种细胞系中，单药紫杉醇与联合用药效果相当，且比单药卡铂更有效。1,25 - D3可能会进一步增强这些药物的细胞毒性作用。
