Department of Obstetrics and Gynecology, Tampere University Hospital, Tampere, Finland
Department of Obstetrics and Gynecology, Tampere University Hospital, Tampere, Finland.
Anticancer Res. 2020 Jun;40(6):3129-3138. doi: 10.21873/anticanres.14294.
BACKGROUND/AIM: The combination of paclitaxel and carboplatin is the standard chemotherapy for ovarian cancer. Previous studies have implied that vitamin D (1,25-D3) may have growth inhibitory effects in ovarian cancer. This study aimed to investigate the effect of paclitaxel, carboplatin and 1,25-D3 on the growth of ovarian cancer cells in vitro, based on the hypothesis that 1,25-D3 might potentiate the effect of paclitaxel and/or carboplatin.
Three non-commercial ovarian carcinoma cell lines UT-OV-1(mucinous), UT-OV-3B (serous) and UT-OV-4 (endometrioid) were exposed to different concentrations of 1,25-D3, paclitaxel and carboplatin, respectively. The cell viability was measured using a Crystal violet assay kit. The cellular vitamin D receptor (VDR) mRNA levels were measured by qRT-PCR using the LightCycler equipment.
The growth-inhibitory effect of the combination of paclitaxel and carboplatin was 56% in UT-OV-1, 33% in UT-OV-3B and 47% in UT-OV-4 cells. Single 1,25-D3 (10 μM) inhibited the growth of UT-OV-3B and UT-OV-4 by 23% and 28%, respectively, whereas no effect was seen in UT-OV-1 cells. These results are in line with the finding that the expression of VDR was high in UT-OV-3B and UT-OV-4, but very low in UT-OV-1. The combination of 1,25-D3, paclitaxel and carboplatin resulted in 61%, 46% and 58% growth reduction in UT-OV-1, UT-OV-3B and UT-OV-4 cells, respectively. The additive effect of 1,25-D3 was 21% in UT-OV-4, 20% in UT-OV-3B and 12% in UT-OV-1 cell line.
The results imply that combining 1,25-D3 with paclitaxel and carboplatin may potentiate their growth inhibitory effect on ovarian cancer cells with high VDR expression.
背景/目的：紫杉醇和卡铂的联合应用是卵巢癌的标准化疗方案。先前的研究表明，维生素 D（1,25-D3）可能对卵巢癌细胞具有生长抑制作用。本研究旨在基于维生素 D（1,25-D3）可能增强紫杉醇和/或卡铂作用的假说，探讨紫杉醇、卡铂和 1,25-D3 对体外卵巢癌细胞生长的影响。
分别用不同浓度的 1,25-D3、紫杉醇和卡铂处理三种非商业性卵巢癌细胞系 UT-OV-1（黏液性）、UT-OV-3B（浆液性）和 UT-OV-4（子宫内膜样）。采用结晶紫试剂盒检测细胞活力。采用 LightCycler 设备的 qRT-PCR 法检测细胞内维生素 D 受体（VDR）mRNA 水平。
紫杉醇和卡铂联合应用对 UT-OV-1、UT-OV-3B 和 UT-OV-4 细胞的生长抑制率分别为 56%、33%和 47%。单独使用 1,25-D3（10 μM）可使 UT-OV-3B 和 UT-OV-4 细胞的生长分别抑制 23%和 28%，而对 UT-OV-1 细胞无影响。这些结果与 VDR 在 UT-OV-3B 和 UT-OV-4 中表达较高，而在 UT-OV-1 中表达较低的发现一致。1,25-D3、紫杉醇和卡铂联合应用可使 UT-OV-1、UT-OV-3B 和 UT-OV-4 细胞的生长分别减少 61%、46%和 58%。1,25-D3 的相加作用在 UT-OV-4 中为 21%，在 UT-OV-3B 中为 20%，在 UT-OV-1 细胞系中为 12%。
结果表明，将 1,25-D3 与紫杉醇和卡铂联合应用可能增强其对高 VDR 表达卵巢癌细胞的生长抑制作用。
