中介亚基MED30在膀胱癌进展中的对比作用
The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer.
作者信息
Syring Isabella, Weiten Richard, Müller Tim, Schmidt Doris, Steiner Susanne, Kristiansen Glen, Müller Stefan C, Ellinger Jörg
机构信息
Clinic for Urology and Paediatric Urology, University Hospital of Bonn, Bonn, Germany
Clinic for Urology and Paediatric Urology, University Hospital of Bonn, Bonn, Germany.
出版信息
Anticancer Res. 2017 Dec;37(12):6685-6695. doi: 10.21873/anticanres.12127.
BACKGROUND/AIM: The Mediator complex is a key regulator of gene transcription, and several studies have demonstrated altered expression of particular subunits in diverse human diseases, especially cancer. To date, nothing is known about the role of MED30 in bladder cancer.
MATERIALS AND METHODS
We, therefore, performed an RNA expression and survival analysis of the subunit MED30 in 537 samples of bladder cancer by using the database cBioPortal. To validate these data on the protein level, we practiced immunohistochemical staining against MED30 on a tissue microarray containing 210 samples of all tumour stages and performed survival analyses. For functional analysis, the siRNA-mediated knockdown of MED30 was performed in the cell lines T24 and TCCSUP followed by proliferation, migration, and invasion assays.
RESULTS
On the mRNA and protein levels, higher expression of MED30 is associated with better patient survival. In accordance with this, advanced T- and N-stages showed lower expression of MED30. In contrast, knockdown of MED30 led to reduction of the tumour parameters proliferation, migration, and invasion in the BCa cell lines.
CONCLUSION
MED30 appears to be integrated in the progression of the urothelial tumour in the bladder.
背景/目的：中介体复合物是基因转录的关键调节因子，多项研究表明，在多种人类疾病尤其是癌症中，特定亚基的表达发生了改变。迄今为止，关于MED30在膀胱癌中的作用尚无相关研究。
材料与方法
因此，我们利用cBioPortal数据库对537例膀胱癌样本中的MED30亚基进行了RNA表达和生存分析。为在蛋白质水平验证这些数据，我们在包含所有肿瘤分期的210个样本的组织芯片上进行了针对MED30的免疫组化染色，并进行了生存分析。为进行功能分析，在T24和TCCSUP细胞系中进行了siRNA介导的MED30敲低，随后进行增殖、迁移和侵袭实验。
结果
在mRNA和蛋白质水平上，MED30的高表达与患者更好的生存相关。与此一致的是，晚期T和N分期显示MED30表达较低。相反，MED30的敲低导致膀胱癌细胞系中的肿瘤参数增殖、迁移和侵袭减少。
结论
MED30似乎参与了膀胱尿路上皮肿瘤的进展。
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