视神经损伤对体外培养的成年大鼠视网膜神经节细胞轴突再生的条件作用。
The conditioning effect of optic nerve injury upon axonal regrowth from adult rat retinal ganglion cells explanted in vitro.
作者信息
Johnson A R, Gregson N A, Wigley C B, Berry M
机构信息
Anatomy Department, United Medical School (Guy's Campus), London, U.K.
出版信息
Neurosci Lett. 1989 Feb 13;97(1-2):63-8. doi: 10.1016/0304-3940(89)90140-7.
An in vitro assay was used to determine the effects of conditioning nerve lesions on the regeneration of adult rat retinal ganglion cell (RGC) axons from retinal explants. Following the conditioning lesion (CL) of unilateral optic nerve transection, maximal regrowth was seen from RGC explanted from ipsilateral retinae 10 days post-CL. Explants from this group initiated axonal regrowth earlier and a greater percentage regrew axons when compared with explants from normal rats. Axonal regrowth from explants of retinae contralateral to CL was also seen earlier than normal. In further experiments, the effects of both exposure of the optic nerve sheath in the orbit and the incision of the dura without injury to optic nerve axons were studied. The conditioning effect of a dural incision was found to be the same as that of optic nerve transection, whilst exposure of the optic nerve sheath had no conditioning effect on RGC axonal regrowth in vitro.
采用体外试验来确定预处理性神经损伤对成年大鼠视网膜外植体中视网膜神经节细胞（RGC）轴突再生的影响。在单侧视神经横断的预处理性损伤（CL）后，在CL后10天，从同侧视网膜外植的RGC可见最大程度的再生。与正常大鼠的外植体相比，该组外植体更早开始轴突再生，且有更大比例的轴突再生。来自CL对侧视网膜外植体的轴突再生也比正常情况更早出现。在进一步的实验中，研究了眼眶内视神经鞘暴露和硬脑膜切开而不损伤视神经轴突的影响。发现硬脑膜切开的预处理效果与视神经横断相同，而视神经鞘暴露在体外对RGC轴突再生没有预处理作用。
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