Xue Langyue, Zeng Yuxiao, Li Qiyou, Li Yijian, Li Zhengya, Xu Haiwei, Yin Zhengqin
Southwest Hospital/Southwest Eye Hospital, Third Military Medical University, Chongqing 400038, China.
Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Chongqing 400038, China.
Oncotarget. 2017 Oct 16;8(54):93087-93102. doi: 10.18632/oncotarget.21857. eCollection 2017 Nov 3.
There is still not an effective treatment for continuous retinal light exposure and subsequent photoreceptor degeneration. Olfactory ensheathing cell (OEC) transplantation has been shown to be neuroprotective in spinal cord, and optic nerve injury and retinitis pigmentosa. However, whether OECs protect rat photoreceptors against light-induced damage and how this may work is unclear. Thus, to elucidate this mechanism, purified rat OECs were grafted into the subretinal space of a Long-Evans rat model with light-induced photoreceptor damage. Light exposure decreased a- and b- wave amplitudes and outer nuclear layer (ONL) thickness, whereas the ONL of rats exposed to light for 24 h after having received OEC transplants in their subretinal space was thicker than the PBS control and untreated groups. A- and b- wave amplitudes from electroretinogram of OEC-transplanted rats were maintained until 8 weeks post OEC transplantation. Also, transplanted OECs inhibited formation of reactive oxygen species in retinas exposed to light. experiments showed that OECs had more total antioxidant capacity in a co-cultured 661W photoreceptor cell line, and cells were protected from damage induced by hydrogen-peroxide. Thus, transplanted OECs preserved retinal structure and function in a rat model of light-induced degeneration by suppressing retinal oxidative stress reactions.
对于持续的视网膜光暴露及随后的光感受器变性，目前仍没有有效的治疗方法。嗅鞘细胞（OEC）移植已被证明在脊髓、视神经损伤和色素性视网膜炎中具有神经保护作用。然而，OECs是否能保护大鼠光感受器免受光诱导损伤以及其作用机制尚不清楚。因此，为了阐明这一机制，将纯化的大鼠OECs移植到光诱导光感受器损伤的Long-Evans大鼠模型的视网膜下间隙。光暴露降低了a波和b波振幅以及外核层（ONL）厚度，而在视网膜下间隙接受OEC移植后再暴露于光下24小时的大鼠的ONL比PBS对照组和未处理组更厚。OEC移植大鼠视网膜电图的a波和b波振幅在OEC移植后8周内保持稳定。此外，移植的OECs抑制了暴露于光下的视网膜中活性氧的形成。实验表明，OECs在共培养的661W光感受器细胞系中具有更高的总抗氧化能力，并且细胞免受过氧化氢诱导的损伤。因此，移植的OECs通过抑制视网膜氧化应激反应，在光诱导变性的大鼠模型中保留了视网膜结构和功能。
