Masuda K, Kinoshita Y, Kobayashi Y
Department of Pediatrics, Kansai Medical University, Osaka, Japan.
Pediatr Res. 1989 Jan;25(1):6-10. doi: 10.1203/00006450-198901000-00002.
Chemotaxis and adherence to polymorphonuclear neutrophils (PMN) subpopulations from cord blood of 13 healthy neonates and blood of 16 healthy adults as control subjects were determined using the rosette-forming procedure, the modified Boyden method, and 51Cr adherence assay. The percentage of rosette-forming neutrophils (RFN) (Fc receptor expression) of cord PMN (35 +/- 8%) was significantly lower than that of adult PMN (60 +/- 4%, p less than 0.01). Differences in chemotaxis of PMN subpopulations between cord and adult PMN after stimulation by their own endotoxin-activated plasma were as follows: (i) unfractionated adult PMN (A) (n = 10) versus adult RFN (B) (n = 5) versus adult nonRFN (C) (n = 5); (A) less than (B), p less than 0.01 and (A) greater than (C), p less than 0.01; (ii) unfractionated cord PMN (a) (n = 5) versus cord RFN (b) (n = 5) versus cord non-RFN (c) (n = 5); (a) less than (b), p less than 0.01 and (a) versus (c), NS; (iii) (A) greater than (a), p less than 0.01; (iv) (B) greater than (b), p less than 0.05 and (C) versus (c), NS. Similarly, differences in adherence were as follows: (i) (A) (n = 13) versus (B) (n = 4), NS and (A) greater than (C) (n = 4), p less than 0.01; (ii) (a) (n = 4) versus (b) (n = 4), NS and (a) greater than (c) (n = 4), p less than 0.01; (iii) (A) greater than (a), p less than 0.05; (iv) (B) versus (b), NS and (C) versus (c), NS. These results suggest that differences in chemotaxis and adherence between cord and adult PMN may relate in part to differences of PMN subpopulations.
采用玫瑰花结形成试验、改良的博伊登方法和51Cr黏附试验，测定了13名健康新生儿脐带血和16名健康成年人血液中的多形核中性粒细胞（PMN）亚群的趋化性和黏附性，后者作为对照对象。脐带血PMN的玫瑰花结形成中性粒细胞（RFN）百分比（Fc受体表达）（35±8%）显著低于成年人PMN（60±4%，p<0.01）。脐带血和成年人的PMN亚群在自身内毒素激活血浆刺激后的趋化性差异如下：（i）未分级的成年人PMN（A）（n=10）与成年人RFN（B）（n=5）与成年人非RFN（C）（n=5）；（A）<（B），p<0.01且（A）>（C），p<0.01；（ii）未分级的脐带血PMN（a）（n=5）与脐带血RFN（b）（n=5）与脐带血非RFN（c）（n=5）；（a）<（b），p<0.01且（a）与（c）比较，无显著性差异；（iii）（A）>（a），p<0.01；（iv）（B）>（b），p<0.05且（C）与（c）比较，无显著性差异。同样，黏附性差异如下：（i）（A）（n=13）与（B）（n=4）比较，无显著性差异且（A）>（C）（n=4），p<0.01；（ii）（a）（n=4）与（b）（n=4）比较，无显著性差异且（a）>（c）（n=4），p<0.01；（iii）（A）>（a），p<0.05；（iv）（B）与（b）比较，无显著性差异且（C）与（c）比较，无显著性差异。这些结果表明，脐带血和成年人PMN在趋化性和黏附性方面的差异可能部分与PMN亚群的差异有关。
